Nutritional evaluation of soybean meal after fermentation with two fish gut bacterial strains, Bacillus cereus LRF5 and Staphylococcus caprae CCF2 in for-mulated diets for Labeo rohita fingerlings

Suhas Kumar Dan, Goutam Banerjee, Ankita Nandi, Arun Kumar Ray

Abstract


Twelve isonitrogenous (35 % crude protein) and isocaloric (18.0 kJ/g) diets were formulated incorporating raw and fermented soybean meal (SBM) at 15%, 30%, 45% and 60% levels by weight. Two phytase-producing bacterial strains, Bacillus cereus LRF5 and Staphylococcus caprae CCF2 isolated from the gut of adult Labeo rohita and Catla catla, respectively were used for fermentation of SBM. Fermentation of SBM was effective in reducing the anti-nutritional factors, trypsin inhibitor and phytic acid and enhancing protein, lipid and mineral concentration. The response of L. rohita, fingerlings (initial weight 3.33±0.07 g) fed the experimental diets for 100 days was compared with fish fed a fish meal based diet. In terms of growth, feed conversion ratio and protein efficiency ratio, diet S7 containing 45% SBM fermented with B. cereus LRF5 resulted in a significantly (P<0.05) better performance of fish. The overall performance of L. rohita fed fermented SBM incorporated diets was better in comparison to those fed raw SBM incorporated diets. The apparent digestibility of nutrients and minerals was significantly (P<0.05) higher in fish fed diet S7. The maximum deposition of protein in the carcass was recorded in fish fed diet S7. Diets containing fermented SBM reduced fecal P levels. The use of this fermented feed will definitely increase the production in fish farm. Furthermore, it will also reduce the production cost, as fish meal protein is costly in the market.


Keywords


Soybean meal; fish gut bacteria; phytase; fermentation; diets; growth performance; Labeo rohita fingerlings

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References


Abdel-Warith AA, Younis EM and Al-Asgah Abdualla N (2013) Influence of Dietary Inclusion of FullFat Soybean Meal and Amino Acids Supplementation on Growth and Digestive Enzymes Activity of Nile Tilapia, Oreochromis niloticus. Turkish Journal of Fisheries and Aquatic Sciences 13: 69–77. doi: 10.4194/1303-2712-v13_1_09

Afinah S, Yazid AM, Anis Shobirin MH and Shuhaimi M (2010) Phytase: application in food industry. International Food Research Journal 17: 13–21.

AOAC (1995) Official Methods of Analysis of AOAC International. In: Cunniff P (ed.), Association of Official Analytical Chemists, 16th edition, AOAC International Arlington, Virginia, USA.

AOAC (Association of Official Analytical Chemists) (1990) Official methods of analysis of the association of official analytical chemists. 1134 pp. In: Helrich W (ed.), Association of Official Analytical Chemists, 15th ed, Washington, DC.

APHA (American Public Health Association) (1985) Standard methods for the examination of water and waste water. 1134 pp. American Water Works Association and Water Pollution. Control Federation, 16th edition. Washington, DC.

Azarm HM and Lee SM (2014) Effects of partial substitution of dietary fish meal by fermented soybean meal on growth performance, amino acid and biochemical parameters of juvenile black sea bream Acanthopagrus schlegeli. Aquaculture Research 45: 994–1003. doi: 10.1111/are.12040

Bairagi A, Ghosh KS, Sen SK and Ray AK (2002) Duckweed (Lemna polyrhiza) leaf meal as a source of feedstuff in formu-lated diets for rohu (Labeo rohita Ham.) fingerlings after fermentation with a fish intestinal bacterium. Bioresource Technology 85: 17–24. doi: 10.1016/s0960-8524(02)00067-6

Baruah K, Pal AK, Sahu NP, Debnath D, Nourozitallab P and Sorgeloos P (2007) Microbial phytase supplementation in rohu, Labeo rohita diets enhances growth performance and nutrient digestibility. Journal of World Aquaculture Society 38: 129–137. doi: 10.1111/j.1749-7345.2006.00081.x

Beutler AL (2009) The Efficacy of Quantum™ Phytase in Laying Hens Fed Corn-Soybean Meal Based Diets. Master of Science (MSc.) Theses, University of Saskatchewan.

Bhargav S, Panda BP, Ali M and Javed S (2008) Solid-state fermentation: an overview. Chemical and Biochemical Engineering Quarterly 22: 49–70.

Biswas AK, Kaku H, Ji SC, Seoka M and Takii K (2007) Use of soybean meal and phytase for partial replacement of fish meal in the diet of red sea bream, Pagrus major. Aquaculture 267: 284–291. doi: 10.1016/j.aquaculture. 2007.01.014

Cheng ZJ (2004) Apparent digestibility of dry matter and nutrients in rainbow trout fed phytase pretreated soybean meal diets. Journal of Feed Corner 16: 32–34.

Cho CY, Slinger SJ and Bayley HS (1982) Bioenergetics of Salmonid fishes: energy intake, expenditure and productivity. Comparative Biochemistry and Physiology part (B) 73: 25–41. doi: 10.1016/0305-0491(82)90198-5

Chou RL, Her BY, Su MS, Hwang G, Wu YH and Chen HY (2004) Substituting fish meal with soybean meal in diets of juvenile cobia Rachycentron canadum. Aquaculture 229: 325–333. doi: 10.1016/s0044-8486(03) 00395-8

Dan SK, Banerjee G, Nandi A, Ghosh P and Ray AK (2015) Au-tochthonous phytase-producing bacteria isolated from the gastrointestinal tracts of four Indian fresh water teleost: characterization and identification. Journal of Microbiology, Biotechnology and Food Sciences 4: 342–346. doi: 10.15414/jmbfs.2015.4.4.342-346

Duncan DB (1955) Multiple range and multiple F-tests. Biometrics 11: 1–42.

Dvorˇa´kova´ J (1998) Phytase: sources, preparation and exploitation. Folia Microbiologica 43: 323–338. doi: 10.1007/bf02818571

El-Saidy DMSD and Gaber MMA (2003) Replacement of fish meal with a mixture of different plant protein sources in juvenile Nile tilapia, Oreochromis niloticus (L.) diets. Aquaculture Research 34: 1119–1127. doi: 10.1046/j.1365-2109.2003.00914.x

El-Sayed AFM (2004) Protein nutrition of farmed tilapia: searching for unconventional sources. PP 364-378. In: Bolivar R, Mair G and Fitzsimmons (eds) New dimensions in farmed tilapia: Proceedings of the Sixth International Symposium on Tilapia in Aquaculture, Manila, Philippines.

Erdman JW Jr and Poneros-Schneier AG (1989) Phytic acid inter-actions with divalent cations in foods and in the gastrointestinal tract. Advances in Experimental Medicine and Bi-ology 249: 161–171. doi: 10.1007/978-1-4684-9111-1_11

Feng J, Liu X, Xu ZR, Lu YP and Liu YY (2007) Effect of fermented soybean meal on intestinal morphology and digestive enzyme activities in weaned piglets. Digestive Diseases and Sciences 52: 1845–1850. doi: 10.1007/ s10620-006-9705-0

Florou-Paneri PC, Christaki E, Giannenas I and Peng J (2014) Alternative protein sources to soybean meal in pig diets. Journal of Food Agriculture and Environment 12: 655–660.

Francis G, Makkar HPS and Becker K (2001) Anti-nutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199: 197–227. doi: 10.1016/s0044-8486(01)00526-9

Francis G, Makkar HPS and Becker K (2001) Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish. Aquaculture 199: 197–227. doi: 10.1016/s0044-8486(01)00526-9

Furuya WM, Pezzato LE, Barros MM, Pezzato AC, Furuya VRB and Miranda EC (2004) Use of ideal protein concept for precision formulation of amino acid levels in fishmeal-free diets for juvenile Nile tilapia (Oreochromis niloticus L.). Aquaculture Research 35: 1110–1116. doi: 10.1111 /j.1365-2109.2004.01133.x

Gatlin DM (2002) Use of soybean meal in the diets of Omnivorous freshwater fish. Department of Wildlife and Fisheries Sciences, Faculty of Nutrition, Texas A&M University System.

Giallongo F, Oh J, Frederick T, Isenberg B, Kniffen DM, Fabin RA and Hristov AN (2015) Extruded soybean meal increased feed intake and milk production in dairy cows. Journal of Dairy Science 98: 6471–85. doi: 10.3168/jds.2015-9786

Greiner R and Konietzny U (2006) Phytase for food application. Food Technology and Biotechnology 44: 125–140.

Hung LT, Thanh NT, Pham MA and Browdy CL (2015) A compari-son of the effect of dietary fungal phytase and dicalcium phosphate supplementation on growth performances, feed and phosphorus utilization of tra catfish juveniles (Pangasianodon hypophthalmus Sauvage, 1878). Aquaculture Nutrition 21: 10–17. doi: 10.1111/ anu.12134

Huynh HPV and Nugegoda D (2011) Effects of Dietary Supplements on Growth Performance and Phosphorus Waste Production of Australian Catfish, Tandanus tandanus, Fed with Diets Containing Soybean Meal as Fishmeal Replacement. Journal of the World Aquaculture Society 42: 645–656. doi: 10.1111/j.1749-7345.2011.00505.x

Kader MA, Koshio S, Ishikawa M, Yokoyama S, Bulbul M, Nguyen BT, Goa J and Laining A (2012) Can fermented soybean meal and squid by-product blend be used as fishmeal re-placements for Japanese flounder (Paralichthys olivaceus)? Aquaculture Research 43: 1427–1438. doi: 10.1111/j.1365-2109.2011.02945.x

Kakade ML, Rackis JJ, McGhee JE and Puski G (1974) Determination of trypsin inhibitor activity of soy products: A collaborative analysis of an improved procedure. Cereal Chemistry 51: 376–382.

Khan MA, Jafri AK, Chadha NK and Usmani N (2003), Growth and body composition of rohu (Labeo rohita) fed diets containing oilseed meals: partial or total replacement of fish meal with soybean meal. Aquaculture Nutrition 9: 391–396. doi: 10.1046/j.1365-2095.2003.00268.x

Liu J, Bollinger DW, Ledoux DR and Veum TL (1998) Lowering the dietary calcium to total phosphorus ratio increases phosphorus utilization in low-phosphorus cornsoybean meal diets supplemented with microbial phytase for growing-finishing pigs. Journal of Animal Science 76: 808–813.

Mondal K, Kaviraj A and Mukhopadhyay PK (2008) Evaluation of fermented fish-offal in the formulated diet of the freshwater catfish Heteropneustes fossilis. Aquaculture Research 39: 1443–1449. doi: 10.1111/j.1365-2109. 2008.02014.x

Mondal K, Kaviraj A, Mukhopadhyay PK, Datta M and Sengupta C (2007) Evaluation of fermented fish offal in formulated diet of the Indian major carp, rohu, Labeo rohita (Hamil-ton). Acta Ichthyologica et Piscatoria 37: 99–105. doi: 10.3750/aip2007.37.2.06

Mukhopadhyay N and Ray AK (1999) Effect of fermentation on the nutritive value of sesame seed meal in the diets for rohu, Labeo rohita (Hamilton), fingerlings. Aquaculture Nutrition 5: 229–236. doi: 10.1046/j.1365-2095. 1999.00101.x

Mukhopadhyay N and Ray AK (2001) Effects of amino acid supplementation on the nutritive quality of fermented linseed meal protein in the diets of rohu, Labeo rohita, fingerlings. Journal of Applied Ichthyology 17: 220–226. doi: 10.1046/j.1439-0426.2001.00287.x

NRC (National Research Council) (1993) Nutrient Requirements Fishes. National Academy Press, Washington DC, USA.

Nwanna LC and Schwarz FJ (2007) Effect of supplemental phytase on growth, phosphorus digestibility and bone mineralization of common carp (Cyprinus carpio L). Aqua-culture Research 38: 1037¬–1044. doi: 10.1111/j.1365 -2109.2007.01752.x

Oliva-Teles A, Pereira JP, Gouveia A and Gomes E (1998) Utilization of diets supplemented with microbial phytase by seabass, Dicentrarchus labrax juveniles. Aquatic Living Resource 11: 255–259. doi: 10.1016/s0990-7440 (98)80008-9

Oser BL (1960) (ed) Hawk’s physiological chemistry. Tata McGraw-Hill Publishing Co. Ltd., New Delhi, 1472 pp.

Papatryphon E and Soares JH (2001) The effect of phytase on apparent digestibility of four practical plant feedstuffs fed to striped bass, Morone saxatilis. Aquaculture Nutrition 7: 161–167. doi: 10.1046/j.1365-2095.2001. 00174.x

Ramachandran S and Ray AK (2007) Nutritional evaluation of fermented black gram (Phaseolus mungo) seed meal in compound diets for rohu, Labeo rohita (Hamilton), fingerlings. Journal of Applied Ichthyology 23: 74–79. doi: 10.1111/j.1439-0426.2006.00772.x

Ramachandran S, Bairagi A and Ray AK (2005) Improvement of nutritive value of grass pea (Lathyrus sativus) seed meal in the formulated diets for rohu, Labeo rohita (Hamilton) fingerlings after fermentation with a fish gut bacterium. Bioresources Technology 96: 1465–1472. doi: 10.1016/j.biortech.2004.12.002

Refstie S, Sahlstrom S, Brathen E, Baeverfjord G and Krogedal P (2005) Lactic acid fermentation eliminates indigestible carbohydrates and antinutritional factors in soybean meal for Atlantic salmon (Salmo salar). Aquaculture 246: 331–345. doi: 10.1016/j.aquaculture.2005. 01.001

Roy T, Banerjee G, Dan SK, Ghosh P and Ray AK (2014) Improvement of nutritive value of sesame oilseed meal in formulated diets for rohu, Labeo rohita (Hamilton), fingerlings after fermentation with two phytase producing bacterial strains isolated from fish gut. Aquaculture International 22: 633–652. doi: 10.1007/s10499-013-9691-0

Roy T, Dan SK, Banerjee G, Nandi A, Ray AK and Ghosh P (2016) Comparative efficacy of phytase from fish gut bacteria and a commercially available phytase in improving the nutritive value of sesame oilseed meal in formulated diets for rohu, Labeo rohita (Hamilton) fingerlings. Acta Icthyologica et Piscatoria 46: 9–23. doi: 10.3750/AIP2016.46.1.02

Simons PC, Versteegh HA, Jongbloed AW, Kemme PA, Slump P, Bos KD, Wolters MG, Beudeker RF and Verschoor GJ (1990) Improvement of phosphorus availability by microbial phytase in broilers and pigs. British Journal of Nutrition 64: 525–540. doi: 10.1079/bjn19900052

Skrede G, Storebakken T, Skrede A, Sahlstrbm S, Sbrensen M, Shearer KD and Slinde E (2002) Lactic acid fermentation of wheat and barley whole meal flours improves digestibility of nutrients and energy in Atlantic salmon (Salmo salar L.) diets. Aquaculture 210: 305–321. doi: 10.1016/s0044-8486(01)00851-1

Spyridakis P, Metailler R, Gabaudan J and Riaza A (1989) Studies on nutrient digestibility in European sea bass (Dicentrarchus labrax). 1. Methodological aspects concerning faeces collection. Aquaculture 77: 61–70. doi: 10.1016/0044-8486(89)90021-5

Steffens W (1989) Principles of fish nutrition. Ellis Horwood, Chichester.

Stein HH, Berger LL, Drackley JK, Fahey Jr GC, Hernot DC and Parsons CM (2008) Nutritional properties and feeding values of soybeans and their coproducts. pp 613-660. In: Johnson LA, White PJ and Galloway RG (eds.), Chemistry, Production, Processing, and Utilization, AOCS Press, Urbana, IL.

Storebakken T, Shearer KD and Roem AJ (1998) Availability of protein, phosphorus and other elements in fishmeal, soy-protein concentrate and hytase-treated soy-protein concentrate-based diets to Atlantic salmon, Salmo salar. Aquaculture 161: 365–379. doi: 10.1016/s0044-8486(97)00284-6

Tharanathan RN and Mahadevamma S (2003) Grain legumesda boon to human nutrition – review. Trends in Food Science and Technology 14: 507–518. doi: 10.1016/j.tifs.2003.07.002

Viola S, Mokady U, Rappapor U and Arieli Y (1982) Partial and complete replacement of fishmeal by soybean meal in feeds for intensive culture of carp. Aquaculture 26: 223–236. doi: 10.1016/0044-8486(82)90158-2

Wheeler EL and Ferrel RE (1971) A method for phytic acid de-termination in wheat and wheat fractions. Cereal Chemistry 48: 312–320.

Wodzinski RJ and Ullah AH (1996) Phytase. Advances in Applied Microbiology 42: 263–302. doi: 10.1016/s0065-2164(08)70375-7

Yang YH, Wang YY, Lu Y and Li QZ (2011) Effect of replacing fish meal with soybean meal on growth, feed utilization and nitrogen and phosphorus excretion on rainbow trout (On-corhynchus mykiss). Aquaculture International 19: 405–419. doi: 10.1007/s10499-010-9359-y

Zambare V (2010) Solid State Fermentation of Aspergillus oryzae for glucoamylase production on Agro residues. International Journal of Life Science 4: 16–25. doi: 10.3126/ijls.v4i0.2892

Zhu Y, Qiu X, Ding Q, Duan M and Wang C (2014) Combined effects of dietary phytase and organic acid on growth and phosphorus utilization of juvenile yellow catfish Pelteobagrus fulvidraco. Aquaculture 430: 1–8. doi: 10.1016/j.aquaculture.2014.03.023

Zhuo L-C, Liu K and Lin Y-H (2014) Apparent digestibility of soy-bean meal and Lactobacillus spp. fermented soybean meal in diets of grouper, Epinephelus coioides. Aquaculture Research 47: 1009–1012. doi: 10.1111/ are.12543




DOI: http://dx.doi.org/10.17017/jfish.v5i1.2017.165

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