Fishing methods in upper Ganga River system of Central Himalaya, India - Journal of Fisheries

Journal of Fisheries
Volume 2 Issue 3 December 2014
Pages: 203-208

Effect of dietary fluted pumpkin (Telfairia occidentalis) extract on growth performance, body composition and haematological parameters of Nile tilapia (Oreochromis niloticus Linnaeus)

Adekunle Ayokanmi Dada • Anike Dolapo Abiodun

Department of Fisheries and Aquaculture Technology, Federal University of Technology, P.M.B. 704, Ondo state, Nigeria

Article type: Original article

DOI: dx.doi.org/10.17017/jfish.v2i3.2014.47

Manuscript history

  • Received on 21 Sep 2014
  • Received in revised form on 22 Nov 2014
  • Accepted on 05 Dec 2014
  • Published online on 07 Dec 2014

Correspondance
Adekunle Ayokanmi Dada
Department of Fisheries and Aquaculture Technology, Federal University of Technology
Email: dadaayokanmi@yahoo.com

Citation
Citation: Dada AA and Abiodun AD (2014) Effect of dietary fluted pumpkin (Telfairia occidentalis) extract on growth performance, body composition and haematological parameters of Nile tilapia (Oreochromis niloticus Linnaeus). Journal of Fisheries 2(3): 203-208. DOI: dx.doi.org/10.17017/jfish.v2i3.2014.47

   
Table of contents
  > Abstract
> Introduction
> Methodology
> Results
> Discussion
> Conclusion
>
References
   
Abstract
The effect of dietary fluted pumpkin extract on growth, body composition and haematological profile was investigated in Nile tilapia Oreochromis niloticus. Fingerlings of about 5.23-5.44 g were fed diets supplemented with four concentrations ((2.5, 5.0, 7.5 and 10.0 gkg−1) of fluted pumpkin extractpowder for eight weeks. Fish fed supplemented diets showed significantly improved growth performance and feed utilization over the control (0 gkg−1 fluted pumpkin extractpowder) treatment. The highest specific growth rate (0.79±0.10 % per day) and best food conversion ratio (0.98±0.14) were obtained in fish fed 2.5 gkg−1 fluted pumpkin extract powder diet. No differences occurred in fish carcass moisture, protein or crude lipid content among the treatments (p>0.05). Similarly no differences occurred in white blood cells among the treatments (p>0.05) but there were greater improvement in the white blood cells of fish fed on dietary fluted pumpkin extractpowder compared to the fish fed the control diet. The results suggest that dietary supplementation with fluted pumpkin extractpowder improved growth rate, feed utilization, white blood cells and survival of Nile tilapia O. niloticus fingerlings.
   
Keywords
Nile tilapia, Oreochromis niloticus, growth, fluted pumpkin extract
 
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Introduction

The cichlid Nile tilapia is the third most important fish species in aquaculture after carp and salmon because of their high protein content and palatability (FAO 2O12) but in spite of the remarkable aquaculture potentials of Nile tilapia, the size of this species obtain under culture is very poor.  Hence, researchers are continually looking for commercial diets that will permit optimal growth of fish without health hazards (Baruah et al. 2008). One of the ways to improve the growth of this fish is through the use of dietary supplements that include plant-based additives. The use of plant-based additives in aquaculture is one of the methods used to improve weight gain, feed efficiency, and/or disease resistance in cultured fish.

Telfairia occidentalis (fluted pumpkin) is cultivated in various parts of southern Nigeria. The darkish green leafy vegetable is used as food and herbal medicine.  The leaf is a rich source of protein, oil, vitamins and minerals but low in crude fibre and also rich source of folic acid, calcium, zinc, potassium, cobalt, copper, iron, vitamins A, C and K (Ajibade et al. 2006).

Fasuyi and Nonyerem (2007) reported that T. occidentalis leafcontains active ingredient which is a plant growth promoter that promotes growth in birds, and this may have similar effects in fish. The leafy vegetables possess anti-microbial and antiviral properties (Nwozo et al. 2004, Olorunfemi et al. 2005).  T. occidentalis extract is also reported to increase hematological parameters (Alada 2000). The objective of this study was to determine the dietary effects T. occidentalis leaf extract powder concentration on growth, body composition and haematological parameters of O. niloticus fingerlings.
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Methodology

Formulation of experimental diets

The Pumpkin leaves were purchased from a market in Akure, Ondo State. The leaves were air dried for 7 days in order to remove the moisture content and the dried materials were milled into flour and later sieved to remove the chaff from the milled leaves. 500 g of the powder (pumpkin leaves) was soaked in distilled water and allowed to ferment naturally at room temperature for 24 hours, After which it was filtered with a muslin bag. The filtrate was evaporated to dryness in water bath to obtain a solid extract. Amounts of 0 (control), 2.5, 5.0, 7.5 and 10 g T. occidentalis extract per kg feed were taken and mixed with a basal feed (30% crude protein), containing fish meal, yellow maize, soybean meal, blood meal, fish oil, vegetable oil, vitamin premix and starch. All dietary ingredients were milled to a 3 mm particle size. The ingredients were thoroughly mixed in a Hobbart A-2007 pelleting and mixing machine (Hobart Ltd, London, UK) to obtain a homogeneous mass, and cassava starch was added as a binder. The resultant mash was pressed without steam through a mixer with a 0.9 mm die. The pellets were dried at ambient temperature (27-30 °C) and stored at −20 °C in a refrigerator. Table 1 shows the proximate composition of the diets.

Experimental procedure

15 (fifteen) plastic tanks (33-litre), each filled with 20 liter of water were aerated continuously using an air compressor. 150 farm-raised O. niloticus fingerling siblings (with an average initial weight of 5.23-5.44 g) were acclimated to laboratory conditions for 14 days at the department of fisheries and aquaculture technology hatchery unit, Federal University of Technology, Akure, Ondo state, Nigeria before being distributed randomly into the fifteen tanks at a stocking density of 15 fish/tank representing five dietary treatments (0, 2.5, 5.0, 7.5 and 10.0 g T. occidentalis leaf extracts per kg feed) designated as FPE1 (control), FPE2, FPE3, FPE4 and FPE5).  Fish were fed at 3% of their body weight (bw) per day in three equal meals, every five hours between 08:00 and 18:00. All fish were weighed and counted fortnightly and feeding rates were adjusted accordingly.

Table 1: Ingredients (g) and proximate composition (%) of feed ingredients
Table 1: Ingredients (g) and proximate composition (%) of feed ingredients

The experiment lasted for 56 days. At the end of the experimental period the following growth and feed utilization parameters were calculated: weight gain (WG), specific growth rate (SGR), food conversion ratio (FCR) and protein efficiency ratio (PER) using the following formulae as described by Brown (1957), Winberg (1956), Castell and Teiws (1980), and Miller and Bender (1955), respectively.

WG = final average weight (g)−initial average weight (g);

SGR (% d–1) = 100 × (ln Wt − ln W0)/t

Where Wt and W0 represent final and initial body weights of fish, respectively, and t represents the duration of the feeding trial;
FCR = dry weight of feed (g) / wet weight gain by fish (g); and PER = wet weight gain by fish (g) / protein intake (g)
Where protein intake (g) = protein (%) in feed × total weight (g) of diet consumed / 100.

Water temperature, pH and dissolved oxygen concentration were routinely monitored in all tanks. At the beginning and end of the feeding trial, pooled samples of 15 fingerlings were analyzed for carcass composition using AOAC (1997) procedures.

Twelve fish (four fish per replicate) were used for blood analysis and 5 ml blood samples from each treatment were collected by cardiac puncture using 5 ml disposable syringes, into treated Bijou bottles. The blood was stored at -40 °C prior to analysis. The blood analysis followed the methods described by Svobodova et al. (1991).

Statistical analyses

The effects of fluted pumpkin leaf extract as feed supplement on growth, haematological parameters were analyzed using one-way analysis (ANOVA) and significant differences among treatment means were compared using Turkey’s multiple range test (Zar 1996).
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Results
 

Mean water quality parameters during the experiment were: dissolved oxygen 7.85±0.5 mgl–1, pH 7.25 and temperature 27.5±0.40 °C. There were improvements in the growth responses of fish fed on T. occidentalis leaf extractsmeal. The best growth responses were obtained in the fish fed on diet FPE2 (2.5 g T. occidentalis leaf extract powder per kg feed) while the slowest growth was obtained in the fish fed diet FPE5 (Table 2).

Table 2: Mean growth performance and feed utilization of O. niloticus fingerlings fed experimental diets for 56 days
Table 2: Mean growth performance and feed utilization of O. niloticus fingerlings fed experimental diets for 56 days

However, there were no significant differences in growth performance across the different T. occidentalis leaf extract powderconcentrations. There were also improvements in the feed conversion ratio (FCR) of fish fed on diets FPE2 and FPE3than the FPE4, FPE5 and FPE1. The average FCRs were 0.98, 1.08, 1.40, 1.47 and 1.19 for diets FPE2, FPE3, FPE4, FPE5 and FPE1 respectively. The PER was 0.06, 0.06, 0.04, 0.05 and 0.05 for the fish fed diets FPE2, FPE3, FPE4, FPE5 and FPE1. There were improvements in the protein content of fish fed on T. occidentalis leaf extract powderthan fish fed the control diet. 

However, there were no significant differences in protein contents across the different T. occidentalis leaf extract powder concentrations and the control diet. The body composition values are given in Table 3. The haematological parameters of O. niloticus (Table 4) showed no significant differences (p≥0.05) in red blood cell, haemoglobin or pack cell volume in all the treatments. However, there was a significant difference (p<0.05) in the white blood cell count of fish in the treatments. Fish fed on diets supplemented with T. occidentalis leaf extract powderhad significantly higher (p<0.05) white blood cell counts.

Table 3: Chemical composition of whole body of O. niloticus fingerlings fed experimental diets (wet basis)
Table 3: Chemical composition of whole body of O. niloticus fingerlings fed experimental diets (wet basis)

Table 4: Some haematological characteristics of O. niloticus fed the experimental diets
Table 4: Some haematological characteristics of O. niloticus fed the experimental diets

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Discussion
 

The results suggest that dietary T. occidentalis leaf extract promoted the growth of O. niloticus fingerlings at 2.5 and 5.0 g/kg feed concentrations; and when the concentration increased to 7.5 and 10.0 g/kg feed, there was no additional growth. This was the first attempt to investigate potential of T. occidentalis leaf extract as a feed additive in aquaculture.

Telfairia occidentalis extract have been reported to increase growth and haematological parameters in rat (Olorunfemi et al. 2005) and poultry birds (Fasuyi and Nonyerem 2007). However, no information was found on the effects of T. occidentalis leaf extract on tilapia survival and growth. The present study revealed that T. occidentalis leaf extract –based diet improve growth and increase the level of white blood cells in Nile tilapia.

These results showed that the T. occidentalis leaf extract in diet enhances nutrient utilization, which is reflected in improved weight gain, FCR and SGR. Generally, low feed conversion ratio were obtained in all treatments, but the poorest occurred in FPE5 (10 g/kg diet) (Table 2). Although better FCR values were obtained in diets FPE2 and FPE3 treatments compared to the control, differences in the treatment means were not significant (p>0.05). Similar results were reported by Turan and Akyurt (2005) who used the extract of red clover Trifolium pratense as a growth-promoting agent for catfish Clarias gariepinus. Immanuel et al. (2009) also reported that tilapia O. mossambicus fingerlings fed on diets supplemented by medicinal plants extracts exhibited faster growth than those fed with the control diet. Similar results were observed using medicinal plants extracts as growth-promoting agents for red sea bream Pagrus major (Ji et al. 2007), African catfish C. gariepinus (Soosean et al. 2010), carp Cyprinus carpio (Pakravan et al. 2012), black tiger shrimp larvae(Sankar et al. 2011) and narrow-clawed crayfish Astacus leptodactylus juveniles(Turan et al. 2012).

Kim et al. (1998) suggested that unknown factors in various medicinal herbs led to favorable results in fish growth trials. The present findings may indicate that availability of bioflavonoids in fluted pumpkinstimulate growth in fish. Therefore, the T. occidentalis leaf extract that promotes growth performance in the cichlid tilapia should be tested for its efficacy to induce efficient and economical propagation in other fish. The greater improvement in feed utilization observed with diets supplemented with 2.5-5.0 g fluted pumpkin leaf extract per kg diet suggested that the addition of T. occidentalis leaf extract powder improved feed utilization.

The whole body composition values obtained in this present study were similar to those reported by Pakravan et al. (2012) and Lee et al. (2012). White blood cell counts was significantly higher (p<0.05) in fish fed 2.5, 5.0 and 7.5 g T. occidentalis leaf extract powder per kg diets. The haematological values obtained in the present study are similar to those reported by Soosean et al. (2010) who used mangosteen (Garcinia mangostana) extract as a feed additive in African catfish C. gariepinus fingerlings. Differences in haematological indices of fish in this study could therefore be ascribed to differences in the dietary inclusions of T. occidentalis leaf extract powder in the diets.
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Conclusion
  It is evident from this study that aqueous extract of T. occidentalis leaf powder could enhance growth and haematological parameters of fish after incorporation in feed at dosages of 2.5-5.0 g/kg feed. It suggests that inclusion of fluted pumpkin in the diet would improve the feed utilization and non-specific immune infections in aquaculture. This study is a preliminary investigation conducted to provide an insight for the use of fluted pumpkin leaf extract as a dietary additive added to fish feed to enhance growth and disease resistance. Future research should focus on the purification of the active compounds and their evaluation in order to improve quality and their usage in culture system.
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References
 

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Alada ARA (2000) The haematological effects of Telfairia occidentalis diet preparation: African Journal of Biomedical Research 3(3): 185-186.

AOAC (Association of Official Analytical Chemists) (1997) Official methods of analysis of AOAC International (16th edn). Arlington, Virginia.

Baruah K, Norouzitallab P, Debnath D, Pal A.K and Sahu NP (2008) Organic acids as non-antibiotic nutraceuticals in fish and prawn feed. Aquaculture Health International12: 4-6. 

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FAO (2012) The state of World Fisheries and Aquaculture. Food and Agricultural Organization of the United Nation. 230 pp.

Fasuyi AO and Nonyerem AD ( 2007) Biochemical, nutritional and haematological implications of Telfairia occidentalis leaf meal as protein supplement in broiler starter diets. African Journal of Biotechnology6: 1055-1063.

Immanuel G, Uma RP, Iyapparaj P, Citarasu T, Peter SM, Babu MM and Palavesam A (2009) Dietary medicinal plant extracts improve growth, immune activity and survival of tilapia Oreochromis mossambicus. Journal of Fish Biology 74(7):1462-75. DOI: 10.1111/j.1095 – 8649.2009.02212x

Ji S, Takaoka O, Jeong G, Lee S, Ishumaru K, Seoka M and Takii K (2007) Dietary medicinal herbs improve growth and some non-specific immunity of red sea bream Pagrus major. Fisheries Science 73(1): 63-69. DOI: 10.1111/j.1444-2906.2007.01302x

Kim DS, Noah CH, Jung SW and Jo JY (1998) Effect of Obosan supplemented diet on growth, feed conversion ratio and body composition of Nile tilapia, Oreochromis niloticus. Journal of Aquaculture11: 83-90.

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Nwozo SO, Adaramoye OA and Ajaiyoba EO (2004) Antidiabetic and hypolipidemic studies of Telfairia occidentalis on alloxan – induced diabetic rats. Nigerian Journal of Natural Products and Medicine.8: 45- 47. DOI: org/10.4314/njnpm.v8il.H811814

Olorunfemi AE, Arnold CI, Emmanuel O, Nkaima N and Akeem A (2005) Hypoglycaemic activity of Telfairia occidentalis in rats. Journal of Pharmaceutical and Bioresources 2: 36-42.

Pakravan S, Hajimoradloo A and Ghorbani R (2012) Effect of dietary willow herb, Epilobium hirsutum extract on growth performance, body composition, haematological parameters and Aeromonas hydrophila challenge on common carp, Cyprinus carpio. Aquaculture Research, 43: 861-869.

Sankar G, Elavarasi A, Sakkaravarthi K and Ramamoorthy K (2011) Biochemical changes and growth performance of black tigher shrimp larvae after using Ricinus communis extract as feed additive. International Journal of PharmTech Research 3(1): 201-208.

Soosean C, Marimuthu K, Sudhakaran S and Xavier R (2010) Effect of mangosteen (Garcinia mangostana L.) extracts as a feed additive on growth and hematological parameters of African catfish (Clarias gariepinus) fingerlings. European Review for Medical and Pharmacological Sciences 14: 605-611.

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