Transmission and pathology of Streptococcus inane in monosex Nile tilapia (Oreochromis niloticus) in aquaculture of Bangladesh

  • Md. Mer Mosharraf Hossain Department of Fisheries and Marine Bioscience, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Amimul Ehsan South-West Area Integrated Water Resources Planning and Management Project, Bangladesh Water Development Board, Bangladesh
  • Md. Anisur Rahman Department of Fisheries and Marine Bioscience, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Monjurul Haq Department of Fisheries and Marine Bioscience, Jessore University of Science and Technology, Jessore-7408, Bangladesh
  • Md. Bazlur Rashid Chowdhury Department of Aquaculture, Bangladesh Agricultural University, Mymensingh, Bangladesh
Keywords: Streptococcosis, Monosex tilapia, histopathology, transmission

Abstract

Streptococcus iniae is a major fish pathogen, recently emergent outbreaks were recorded in commercially cultured monosex Nile tilapia (Oreochromis niloticus) result in significant losses termed “streptococcosis”-causes unusual appearances with multi-focal pin-point haemorrhages, abscesses, necrosis and ascites in skin, fin, muscle, liver, spleen, kidney, blood, interstitial fluid specially in central nervous system and brain. This disease was more prevalent (>26%) at summer when the water temperature was approximately >25oC, percentage of mortality was higher >41% during the overcrowding and improper water chemistry. Raised levels of glucose and ammonium in blood serum causes reduced number of free blood cells released into the haemolymph to stomach and gut, result in refrain from eating in diseased tilapia. Stocking density (200 fish/decimal; class IV) had significant effect (P<0.01) on the total production (5,000 to 5,500 kg/ha). S. iniae in the circulating blood cells, extra-tubular haemal spaces containing blood vessels, fixed phagocytes in the hepatopancreas (gastrointestinal tract), bacteria-like particles in the brain tissue, vacuum and necrosis in hepatocytes revealed with histopathology. In vitro study revealed that cohabitation of dead or infected fish with healthy fish resulted infection (horizontal transmission mechanism) to the healthy fish.

References

Abutbul S, Golan-Goldhirsh A, Barazani O and Zilberg D (2004) Use of Rosmarinus officinalis as a treatment against Streptococcus iniae in tilapia (Oreochromis sp.). Aquaculture 238(1-4): 97-105. DOI: 10.1016/j.aquaculture.2004.05.016

Apha (1998) Standard Methods for the Examination of Water and Wastewater. 20th edn. APHA (American Public Health Association), Washington, USA.

Bergmeyer HV (1984) Methods of Enzymatic Analysis. Metabolites I: Carbohydrates, 3rd edition. Weinheim: Verlag Chemie.

Berridge B, Fuller JD, de Azavedo J, Low DE, Bercovier H and Frelier PE (1998) Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer. Journal of Clinical Microbiology 36(9): 2778-2781.

Bolz DF and Howel JA (1978) Colorimetric Determination of Non-Metals. London: Wiley.

Bromage ES and Owens L (2002) Infection of barramundi Lates calcarifer with Streptococcus iniae: Effect of different routes of exposure. Diseases of Aquatic Organisms 52(3): 199-205.

Bromage ES, Thomas A and Owens L (1999) Streptococcus iniae, a bacterial infection in barramundi Lates calcarifer. Diseases of Aquatic Organisms 36(3): 177-181.

Bunch EC and Bejerano I (1997) The effect of environmental factors on the susceptibility of hybrid tilapia Oreochromis niloticus; Oreochromis aureus to streptococcosis. The Israeli Journal of Aquaculture-Bamidgeh 49: 67-76.

Chakraborty SB and Banerjee S (2009) Culture of monosex Nile tilapia under different traditional and non-traditional methods in India. World Journal of Fish and Marine Sciences 1(3): 212-217.

Chakraborty SB, Sarbajna A, Mazumdar D and Banerjee S (2007) Effects of differential dose and duration of 17-methyltestosterone treatment on sex reversal of Nile tilapia, Oreochromis niloticus at different age groups under Indian perspective. Asian Journal of Microbiology, Biotechnology & Environmental Sciences 9(3): 705-710.

Chang PH and Plumb JA (1996) Histopathology of experimental Streptococcus sp. infection in tilapia, O. niloticus and channel catfish, Ictalarus punctatus. Journal of Fish Diseases 19: 235-241.

Diana JS, Yi Y and Lin CK (2004) Stocking densities and fertilization regimes for Nile tilapia (Oreochromis niloticus) production in ponds with supplemental feeding, In: Proceedings of the sixth international symposium on tilapia in aquaculture, R. Bolivar, G. Mair and K. Fitzsimmons, Eds. Manila, Philippines, BFAR, Philippines, pp. 487-499.

Duncan DB (1955) Multiple range and multiple F tests. Biometrics 11: 1-42.

El-Sayed AM (2002) Effect of stocking density and feeding levels on growth and feed efficiency of Nile tilapia (Oreochromis niloticus L.) fry. Aquaculture Research 33(8): 621-626. DOI: 10.1046/j.1365-2109.2002.00700.x

Eldar A, Horovitcz A and Bercovier H (1997) Development and efficacy of a vaccine against Streptococcus iniae infection in farmed rainbow trout. Veterinary Immunology and Immunopathology 56(1-2): 175-183.

Eldar A, Bejerano Y and Bercovier H (1994) Streptococcus shiloi and Streptococcus difficile, two new streptococcal species causing a meningoecephalitis in fish. Current Microbiology 28(3): 139-143. DOI: 10.1007/BF01571054

Eldar A, Bejerano Y, Livoff A, Horovitez A and Bercovier H (1995) Experimental streptococcal meningo-encephalitis in cultured fish. Veterinary Microbiology 43(1): 33-40.

Evans JJ, Klesius PH, Gilbert PM, Shoemaker CA, Al-Sarawi MA, Landsberg J, Durendez R, Al-Marzouk A and Al-Zenki S (2002) Characterization of β-hemolytic group B Streptococcus agalactiae in cultured seabream, Sparus auratus and and mullet, Liza klunzingeri, in Kuwait. Journal of Fish Diseases 25: 505-513.

Facklam R, Elloitt J, Shewmaker L and Reingold A (2005) Identification and characterization of sporadic isolates of Streptococcus iniae from human. Journal of clinical microbiology 43(2): 933-937.

Ferguson HW, St Johns VS, Roach CJ, Willoughby S, Parker C and Ryan R (2000) Caribbean reef fish mortality associated with Streptococcus iniae. The Veterinary Record 147(23): 662-664.

Haylor GS and Pascual AB (1991) Effect of using ram testes in a fry diet for Oreochromis niloticus (L.) on growth, survival and resultant phenotypic sex ratio. Aquatic Fisheries Management 22: 265-268.

Kaige N, Miyazaki T and Kubta SS (1984) The pathogen and histophatology of vertebral deformity in cultured yellowtail. Fish Pathology 19: 173-179.

Kim JH, Gomez DK, Choresca CH and Park SC (2007) Detection of major bacterial and viral pathogens in trash fish used to feed cultured flounder in Korea. Aquaculture 272(1-4): 105-110. DOI: 10.1016/j.aquaculture.2007.09.008

Kitao T, Aoki T and Sakoh R (1981) Epizootic caused by β-haemolytic Streptococcus species in cultured freshwater fish. Fish Pathology 15: 301-307.

Kvitt K and Colorni A (2004) Strain variation and geographical endemism in Streptococcus iniae. Diseases of Aquatic Organisms 61(1-2): 67-73.

Lafferty KD, Porter JW and Ford SE (2004) Are diseases increasing in the ocean? Annual Review of Ecology, Evolution, and Systematic 35: 31-54. DOI: 10.1146/annurev.ecolsys.35.021103.105704

Le Moullac G and Haffner P (2000) Environmental factors affecting immune responses in Crustacea. Aquaculture 191(1-3): 121–131. DOI: 10.1016/S0044-8486(00)00422-1

Lehane L and Rawlin GT (2000) Topically acquired bacterial zoonoses from fish: a review. Journal of the Australian Medical Association 173(5): 256-259.

Mehrim AI, Abdelhamid AM, Abu Shousha AAM, Salem MFI and El-Sharawy MAMM (2006) Nutritious attempts to detoxify aflatoxic diets of tilapia fish: 2- Clinical, biochemical and histological parameters. Journal of Arabic and Aquaculture Society 1: 69-90.

Nguyen HT, Kanai K and Yoshikoshi K (2002) Ecological investigation of Streptococcus iniae in cultured Japanese flounder (Paralichthys olivaceus) using selective isolation procedures. Aquaculture 205(1-2): 7-17. DOI: 10.1016/S0044-8486(01)00667-6

Pechsiri J and Yakupitiyage A (2005) A comparative study of growth and feed utilization efficiency of sex-reversed diploid and triploid Nile tilapia, Oreochromis niloticus L. Aquaculture Research 36(1): 45-51. DOI: 10.1111/j.1365-2109.2004.01182.x

Perera RP, Johnson SK and Lewis DH (1997) Epizootiological aspects of Streptococcus iniae affecting tilapia in Texas. Aquaculture 152(1-4): 25-33. DOI: 10.1016/S0044-8486(96)01450-0

Pier GB and Madin SH (1976) Streptococcus iniae, a beta-hemolytic Streptococcus isolated from Amazon freshwater dolphin, Inia geoffrensis. International Journal of Systematic Bacteriology 26: 545-553.

Plumb JA (1997) Infectious diseases of tilapia. In: Costa-Pierce, B.A., Rakocy, J.E. (Eds.), Tilapia Aquaculture in the Americas vol. 1 World Aquaculture Society, Baton Rouge, LA, USA, pp. 212-218.

Popma TJ and Green BW (1990) Sex reversal of tilapia in earthen ponds. Research and Development Series No. 35. International Center for Aquaculture, Alabama Agricultural Experiment Station, Auburn University, AL, USA.

Robinson JA and Meyer FP (1966) Streptococcal fish pathogen. Journal of Bacteriology 92(2): 512.

Shelton WL, Hopkins KD and Jensen GL (1978) Use of hormones to produce monosex tilapia for aquaculture. In The Second International Symposium on Tilapia in Aquaculture. ICLARM Conference Proceedings 15, Eds., Pullin, R.S.V., T. Bhukaswan, K. Tonguthai and J.L. Maclean. Department of Fisheries, Bangkock, Thailand and International Center for Living Aquatic Resources Management, Manila, Philippines, pp: 10-33.

Shen ZH, Qian D, Xiu WJ, Gu JH and Shao JZ (2005) Isolation, identification and pathogenicity of Streptococcus iniae isolated from red drum Sciaenops ocellatus. Acta Hydrobiologica Scadanavika 29: 678-683.

Shoemaker C and Klesius P (1997) Streptococcal disease problems and control: a review. In: Fitzsimmons, K. (Ed)., Tilapia Aquaculture vol. 2 Northeast Regional Agricultural Engineering Service-106, Ithaca, NY, USA, pp. 671-680.

Shoemaker CA, Evans JJ and Klesius PH (2000) Density and dose: factors affecting mortality of Streptococcus iniae infected tilapia (Oreochromis niloticus). Aquaculture 188: 229-235.

Shoemaker CA, Klesius PH and Evans JJ (2001) Prevalence of Streptococcus iniae in tilapia, hybrid striped bass, and channel catfish on commercial fish farms in the United States. American Journal of Veterinary Research 62(2): 174-177

Stoffregen DA, Backman SC, Perham RE, Bowser PR and Babish JG (1996) Initial disease report of Streptococcus iniae infection in hybrid striped (sunshine) bass and successful therapeutic intervention with fluoroquinolone antibacterial enerofloxacin. Journal of the World Aquaculture Society 27: 420-436.

Tran-Duy A, Schrama JW, Van Dam AA, Verreth JAJ (2008) Effects of oxygen concentration and body weight on maximum feed intake, growth and hematological parameters of Nile tilapia, Oreochromis niloticus. Aquaculture 275(1-4):152-162. DOI: 10.1016/j.aquaculture.2007.12.024

Van Handel E (1965) Microseparation of glycogen, sugars and lipids. Analytical Biochemistry 11(2): 266-271. DOI: 10.1016/0003-2697(65)90014-X

Xu DH, Shoemaker CA and Klesius PH (2007) Evaluation of the link between grodactylosis and Streptococcus of Nile tilapia (O. niloticus). Fish Diseases 30: 230-238.

Yoganandhan K, Thirupathi S and Sahul Hameed AS (2003) Biochemical, physiological and haematological changes in white spot syndrome virus-infected shrimp, Penaeus indicus. Aquaculture 221(1-4): 1-11. DOI: 10.1016/S0044-8486(02)00220-X

Zar JH (1984) Biostatistical analysis. Prentice-Hall Inc., Englewood Cliffs, New Jersey, USA.

Zlotkin A, Hershko H and Eldar A (1998) Possible transmission of Streptococcus iniae from wild fish to cultured marine fish. Applied and Environmental Microbiology 64(10): 4065-4067.

Published
2014-04-30
How to Cite
Hossain, M. M. M., Ehsan, A., Rahman, M. A., Haq, M., & Chowdhury, M. B. R. (2014). Transmission and pathology of Streptococcus inane in monosex Nile tilapia (Oreochromis niloticus) in aquaculture of Bangladesh. Journal of Fisheries, 2(1), 90-99. https://doi.org/10.17017/j.fish.66