New host and geographical record of Eudactylina pusilla Cressey, 1967 from Indian waters with DNA barcodes

  • Pradeep Hosahalli Divakar Fishery Survey of India, Mormugao Zonal Base, Mormugao 403803, Goa, India
  • Venu Sasidharan Department of Oceanography & Marine Biology, Brookshabad Campus, Pondicherry University, PBNo.1, Chakkargaon P.O., Port Blair 744112, Andaman and Nicobar Islands, India
  • Ravi Ranjan Kumar Department of Oceanography & Marine Biology, Brookshabad Campus, Pondicherry University, PBNo.1, Chakkargaon P.O., Port Blair 744112, Andaman and Nicobar Islands, India
  • Sumitha Gopalakrishnan Department of Aquaculture and Fishery Microbiology, Research Centre Calicut University, MES Ponnani College, Ponnani, Malappuram, Kerala, India
  • Bineesh Kinattum Kara Zoological Survey of India, ANRC, 11, Horticulture Road, Haddo, Port Blair 744102, Andaman & Nicobar Islands, India
  • Mahesh Kumar Farejiya Fishery Survey of India, Plot No. 2A, Unit No. 12, New Fishing Harbour, Sassoon Dock, Colaba, Mumbai 400005, India
Keywords: First record, Eudactylina pusilla, pelagic thresher shark, Siphonostomatoida, gill parasites


The present paper reports the first record of the parasite Eudactylina pusilla Cressey, 1967 from the gills of the pelagic thresher shark, Alopias pelagicus Nakamura, 1935 collected during a multifilament longline operation at a depth of 762 m from Indian EEZ around Andaman Islands. The occurrence of this copepod gill parasite on A. pelagicus in the Indian waters constitutes new host record and extends the parasite’s known geographical distribution, thus contributing to the knowledge of biodiversity of the parasitic copepods in Indian waters. Molecular marker based taxonomical annotation using Mitochondrial 18S r DNA sequencing also confirmed the identity of the E. pusilla specimen.


Aneesh PT, Sudha K, Arshad K, Anilkumar G and Trilles JP (2013) Seasonal fluctuation of the prevalence of cymothoids representing the genus Nerocila (Crustacea, Isopoda), parasitizing commercially exploited marine fishes from the Malabar Coast, India. Acta parasitologica 58(1): 80–90.

Anon (2013) Forest Statistics. Department of Environment and Forests, Andaman and Nicobar Islands. 141 pp.

Boxshall G and Halsey S (2004) An introduction to copepod diversity. Ray Society, London 421 pp.

Caira JN (1990) Metazoan parasites as indicators of elasmobranch biology. NOAA Technical Reports, NMFS 90: 71–96.

Caira JN and Healy CJ (2004). Elasmobranchs as hosts of metazoan parasites. pp. 523–551. In: The biology of sharks and their relatives. Carrier J, Musack J, Heithaus E (Eds). CRC Press. Boca Raton.

Caira JN, Mega J and Ruhnke TR (2005) An unusual blood sequestering tapeworm (Sanguilevator yearsleyi n. gen., n. spp.) from Borneo with description of Cathetocephalus resendezi n. spp. from Mexico and molecular support for the recognition of the order Cathetocephalidea (Platyhelminthes: Eucestoda). International Journal of Parasitology 35: 1135–1152.

Compagno JVL (1984) FAO species catalogue Vol. 4, part 2 sharks of the world: an annotated and illustrated catalogue of shark species known to date. FAO, UN.

Cressey RF (1967) Caligoid copepods parasitic on sharks in the Indian Ocean. Proceedings of United States Natural Museum 121(3572): 1–21 (Figs. 1–54).

Cressey RF (1970) Copepods parasites on sharks from the west coast of Florida. Smithson Contribution to Zoology 38: 1–30 (Figs. 1–110).

Deets GB (1994) Copepod–chondrichthyan coevolution: a cladistic consideration. PhD thesis, University of British Columbia.

Dippenaar SM (2009) Estimated molecular phylogenetic relationships of six siphonostomatoid families (copepoda) symbiotic on elasmobranchs. Crustaceana 82(12): 1547–1567.

Dippenaar SM (2016) Biodiversity and studies of marine symbiotic siphonostomatoids off South Africa. African Journal of Marine Sciences 38(1): 1–5.

Dippenaar SM, Van Tonder RC and Winter SP (2009) Is there evidence of niche restriction in the spatial distribution of Kroyeria dispar Wilson, 1935, K. papillipes Wilson, 1932 and Eudactylina pusilla Cressey, 1967 (Copepoda: Siphonostomatoida) on the gill filaments of tiger sharks Galeocerdo cuvier off KwaZulu-Natal, South Africa. Hydrobiologia 619(1): 89–101.

Eiras JC, Pavanelli GC, Takemoto RM and Doencas de Peixes (2000) Profilaxia, diagnostic tratamento. Parana: Editorada Universidad Estadual de Maringa; Portuguese 264 pp.

Francis-Floyd R and Floyd MR (2011) Amyloodinium ocellatum, an important parasite of cultured marine fish. SRAC Publication No. 4705: 1–12.

Helna AK, Sudha K, Aneesh PT, Arshad K and Anilkumar G (2012) Diversity of Bomolochus spp. (Copepoda, Bomolochidae) infesting the edible marine fishes along the Malabar Coast. In: Proceedings of National Conference on Aquatic Animal Health and Management-2012 (NCAAHM-2012), 14–15 September 2012. Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, pp. 52–53.

Huys R, Llewellyn-Hughes J, Conroy-Dalton S, Olson PD, Spinks JN and Johnston DA (2007) Extraordinary host switching in siphonostomatoid copepods and the demise of the Monstrilloida: integrating molecular data, ontogeny and antennulary morphology. Molecular Phylogenetic Evolution 43: 368–378.

Izawa K (2011) Five new species of Eudactylina van Beneden, 1853 (Copepoda, Siphonostomatoida, Eudactylindae) parasitic on Japanese elasmobranchs. Crustaceana 84: 1605–1634.

Jithin K, Swapna A, Kumar RR, Venu S, Helna AK and Sudha K (2016) Studies on crustacean parasites from commercial marine fish along the Andaman Coast in comparison with Malabar Coast of Kerala of Indian EEZ. World Journal of Fisheries and Marine Sciences 8(1): 47–53.

Kabata Z (1979) Parasitic copepoda of British fishes. Ray Society, London. 468 pp. (2031 Figs).

Lebepe MC and Dippenaar SM (2013) A report of symbiotic Siphonostomatoida (Copepoda) infecting mobulids (Rajiformes: Mobulidae) off the KwaZulu-Natal coast, South Africa. African Zoology 48(2): 326–332.

Montesa MM, Castro-Romerob R and Martorellia SR (2017) Morphological identification and DNA barcoding of a new species of Parabrachiella (Siphonostomatoida: Lernaeopodidae) with aspects of their intraspecific variation. Acta Tropica 173: 34–44.

Morales-Serna FN, Pinacho-Pinacho CD, Gómezc S and de Leóna GP (2014) Diversity of sea lice (Copepoda: Caligidae) parasitic on marine fishes with commercial and aquaculture importance in Chamela Bay, Pacific coast of Mexico by using morphology and DNA barcoding, with description of a new species of Caligus. Parasitology International 63: 69–79.

Nashad M, Sijo PV, Swapnil SS and Das P (2018) New records of four parasitic copepods (Crustacea, Siphonostomatoida) from Andaman and Nicobar Waters, India. Journal of Fisheries and Environment 42: 1–11.

Nowak BF (2007) Parasitic diseases in marine cage culture: an example of experimental evolution of parasites. International Journal of Parasitology 37: 581–588.

Palm HW (2011) Fish parasites as biological indicators in a changing world: can we monitor environmental impact and climate change? pp. 223–250. In: Mehlhorn H (Eds) Progress in Parasitology, Research Monographs 2, Springer-Verlag Berlin Heidelberg.

Pillai NK (1968) Additions to the copepod parasites of South Indian fishes. Parasitology 58: 9–36.

Pillai NK (1985) The fauna of India. Copepod parasites of marine fishes. ZSI, Kolkata, India.

Pradeep HD, Arun Kumar M and Swapnil SS (2017a) First report of Echthrogaleus denticulatus (Smith 1874) on the pelagic thresher shark (Alopias pelagicus Nakamura 1935) from Indian EEZ of Andaman Sea. Sains Malaysiana 46(10): 1675–1678.

Pradeep HD, Shirke SS and Devi SM (2016) Report of epizootic cirripede, Conchoderma virgatum (Spengler, 1790) on Pennella instructa (Wilson, 1917) parasitic on Indo-Pacific sailfish Istiophorus platypterus caught from Andaman Sea. Journal of Environmental and Zoological Studies 4 (4): 1208–1210.

Pradeep HD, Shirke SS, Nashad M and Devi SM (2017b) New host and geographical record of the pandarid copepod, Pandarus cranchii (Leach, 1819) on the pelagic thresher shark, Alopias pelagicus Nakamura, 1935 from Andaman Sea. Journal of Environment and Zoology Studies 5(1): 834–838.

Pradeep HD, Venu S and Sumitha G (2019) First record of Gloiopotes watsoni Kirtisinghe, 1934 parasitic on Istiophorus platypteurs (Shaw and Nodder 1792) from Andaman Sea with DNA barcodes. I3 Biodiversity 5: 1–8.

Pradeep HD, Venu S, Sumitha G, Shirke SS and Bineesh KK (2018) First report of Tristomella laevis (Verrill, 1875) Guiart, 1938 parasitic on Istiophorus platypterus (Shaw and Nodder, 1792) from Andaman Sea. Indian Journal of Geo-Marine Sciences 47(8): 1616–1619.

Ramesh Kumar G and Ravichandran S (2013) Effect of the parasitic isopod, Catoessa boscii (Isopoda, Cymothoidae), a buccal cavity parasite of Carangoides malabaricus. Asian Pacific Journal of Tropical Biomedicine 3(2): 118–122.

Randhawa HS and Poulin R (2010) Determinants of tapeworm species richness in elasmobranch fishes: untangling and phylogenetic influences. Ecography 33: 866–877.

Rangnekar MP (1956) Parasitic copepods from the marine fishes of Bombay. Journal of University of Bombay 24: 42–65.

Rangnekar MP (1957) Copepod parasites of the families Argulidae, Caligidae, Dichelesthiidae and Lernaeopodidae. Journal of University of Bombay 26: 8–20.

Ravichandran S, Ramesh Kumar G and Kumaravel K (2009) Variation in the morphological features of isopod fish parasites. World Journal of Fisheries and Marine Sciences 1(2): 137–140.

Reardon M, Márquez F, Trejo T and Clarke SC (2009) Alopias pelagicus. The IUCN red list of threatened species 2009: e.T161597A5460720 (accessed on 19 December 2018).

Salmon ER (2015) Spatial sistribution of Eudactylina spp. (Copepoda: Siphonostomatoida: Eudactylinidae) infecting gill of angel sharks (Squatina spp.) captured in the northeastern Gulf of Mexico. Doctoral dissertation, Middle Tennessee State University.

Tamura K and Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512–526.

Tamura K, Stecher G, Peterson D, Filipski A and Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729.

Walter TC and Boxshall G (2018) World of copepods database. Eudactylina pusilla Cressey, 1967. (accessed on 2018-08-15).

Watchariya P, Phithak C and Thanitha Thapanand C (2009) Parasites of deep-sea sharks from the Andaman Sea with six new records of parasites in Thailand. Kasetsart Journal (Natural Science) 43: 93–99.

How to Cite
Divakar, P. H., Sasidharan, V., Kumar, R. R., Gopalakrishnan, S., Kara, B. K., & Farejiya, M. K. (2019). New host and geographical record of Eudactylina pusilla Cressey, 1967 from Indian waters with DNA barcodes. Journal of Fisheries, 7(2), 700-705. Retrieved from