The effect of different dietary protein levels on the water quality and reproductive performance of Nile tilapia (Oreochromis niloticus) broodstock in biofloc system
Abstract
This study investigated the effect of protein levels on the water quality and reproductive performance of Nile tilapia (Oreochromis niloticus) in the biofloc system. Three isocaloric diets with different protein levels (32%, 38%, and 44%) were formulated. A total of nine one-tone tanks with a water holding capacity of 0.9 tons were prepared and equipped with the necessary materials to form biofloc. Seventy-two female and thirty-six male fish were tagged, numbered, and randomly released into the tanks at a female: male ratio of 2:1. The results showed that the lowest amount of TAN (0.21 mgL–1), NO2, and NO3 were observed in the treatment of 32% protein. Mean final weight in the 32% and 38% treatments was 350 and 356 g, respectively, with no significant difference. The highest absolute fecundity (654) was observed in the 32% group, but no significant difference was found between the absolute fecundity of the 38% and 44% groups. The longest interval (17.8 days) was observed in the 44% protein group. The percentage of fertilization and hatching did not significantly differ between the groups. The highest percentage of fertilization (93%) and hatching (89%) were observed in the 32% protein group. The total number of eggs per female fish did not differ between the 32% (12840) and 38% (12670) groups, while the 44% group had the lowest number of eggs (12040). The present study showed that a dietary protein level of 32% is optimal for water quality and reproductive performance of Nile tilapia in the biofloc system.
References
Ali M, Wootton R (1999) Effect of variable food levels on reproductive performance of breeding female three‐spined sticklebacks. Journal of Fish Biology 55: 1040–1053.
AOAC (2023) Association of Official Analytical Collaboration Official Method 923.03. Ash of Flour: Direct Method. In: Latimer Jr. GW (Ed) Official methods of analysis of AOAC, 22nd edition, Oxford University Press, C32-2.
APHA (2005) Standard methods for the examination of water and wastewater, 21st edition. American Public Health Association/American Water Works Association/Water Environment Federation, Washington DC.
Arshad M, Ali S, Afzal W, BiBi Z, Mohsin M, ... Sabtain T (2024) Impact of various dietary protein sources on growth performance of Nile Tilapia (Oreochromis niloticus) fingerlings and on water quality. Haya: Saudi Journal of Life Sciences 9: 369–376.
Avnimelech Y (2009) Biofloc technology: a practical guide book, World Aquaculture Society, Louisiana, USA.
Bhujel RC (2000) A review of strategies for the management of Nile tilapia (Oreochromis niloticus) broodfish in seed production systems, especially hapa-based systems. Aquaculture 181: 37–59.
Binalshikh-Abubkr T, Hanafiah MM, Das SK (2021) Proximate chemical composition of dried shrimp and tilapia waste bioflocs produced by two drying methods. Journal of Marine Science and Engineering 9: 193.
Bombardelli RA, dos Reis Goes ES, de Negreiros Sousa SM, Syperreck MA, Goes MD, ... Meurer F (2017) Growth and reproduction of female Nile tilapia fed diets containing different levels of protein and energy. Aquaculture 479: 817–823.
Cardona E, Lorgeoux B, Chim L, Goguenheim J, Le Delliou H, Cahu C (2016) Biofloc contribution to antioxidant defence status, lipid nutrition and reproductive performance of broodstock of the shrimp Litopenaeus stylirostris: consequences for the quality of eggs and larvae. Aquaculture 452: 252–262.
de Alba G, Sánchez-Vázquez FJ, López-Olmeda JF (2021) Reproductive physiology of tilapia. Biology and aquaculture of Tilapia. CRC Press Taylor & Francis Group. pp. 157–177.
de Oliveira MM, Ribeiro T, Orlando TM, de Oliveira DGS, Drumond MM, ... Rosa PV (2014) Effects crude protein levels on female Nile tilapia (Oreochromis niloticus) reproductive performance parameters. Animal Reproduction Science 150: 62–69.
Ekasari J, Suprayudi MA, Wiyoto W, Hazanah RF, Lenggara GS, ... Zairin JM (2016) Biofloc technology application in African catfish fingerling production: the effects on the reproductive performance of broodstock and the quality of eggs and larvae. Aquaculture 464: 349–356.
Ekasari J, Zairin M, Putri DU, Sari NP, Surawidjaja EH, Bossier P (2015) Biofloc-based reproductive performance of Nile tilapia Oreochromis niloticus L. broodstock. Aquaculture Research 46: 509–512.
El-Sayed AFM (2021) Use of biofloc technology in shrimp aquaculture: a comprehensive review, with emphasis on the last decade. Reviews in Aquaculture 13: 676–705.
El-Sayed AFM, Kawanna M (2008) Optimum water temperature boosts the growth performance of Nile tilapia (Oreochromis niloticus) fry reared in a recycling system. Aquaculture Research 39: 670–672.
El-Sayed AFM, Mansour CR, Ezzat AA (2003) Effects of dietary protein level on spawning performance of Nile tilapia (Oreochromis niloticus) broodstock reared at different water salinities. Aquaculture 220: 619–632.
Emerenciano M, Cuzon G, Arévalo M, Gaxiola G (2014) Biofloc technology in intensive broodstock farming of the pink shrimp Farfantepenaeus duorarum: spawning performance, biochemical composition and fatty acid profile of eggs. Aquaculture Research 45: 1713–1726.
Engdaw F, Geremew A (2024) Broodstock nutrition in Nile tilapia and its implications on reproductive efficiency. Frontiers in Aquaculture 3: 1281640.
Freato TA, Freitas RTF, Pimenta MESG, Oliveira GR, Reis Neto RV, Mattos BO (2012) Evaluation of Nile tilapia strains cultivated in cages under different feeding programmes. Revista Brasileira de Zootecnia 41: 1332–1336.
Fülber VM, Ribeiro RP, Vargas LD, Braccini GL, Marengoni NG, de Godoy LC (2010) Desempenho produtivo de três linhagens de tilápia-do-Nilo (Oreochromis niloticus) alimentadas com dois níveis de proteína. Acta Scientiarum. Animal Sciences 32: 77–83 (in Portuguese).
Gamboa‐Delgado J, Márquez‐Reyes JM (2018) Potential of microbial‐derived nutrients for aquaculture development. Reviews in Aquaculture 10: 224–246.
Ghaedi A, Hosseinzadeh H, Hashim R (2019) Effect of different protein levels on reproductive performance of snakehead murrel Channa striatus (Bloch 1793). Iranian Journal of Fisheries Sciences 18: 812–829.
Ghaedi A, Kabir MA, Hashim R (2013) Oocyte development and fecundity of snakehead murrel, Channa striatus (Bloch 1793) in captivity. Asian Fisheries Science 26: 39–51.
Ghaedi A, Sarsangi H, Akhavan M, Ali Mahmudi M, Mohamadi M (2022) Biofloc system preparation in simple language. Advanced Aquaculture Sciences Journal 6: 51–61.
Ghimire RR, Ghimire A, Karki D, Basyal D, Rai KB (2023) Determination of ammonia level and its protein conversion in the water of biofloc fish farming technology. Prithvi Academic Journal 6: 11–20.
Green BW, Rawles SD, Ray CL, McEntire ME (2024) Relationship between stocking rate and production of stocker hybrid tilapia and water quality in a mixtotrophic biofloc system. Journal of the World Aquaculture Society 55: e13087.
Hafedh YA (1999) Effects of dietary protein on growth and body composition of Nile tilapia, Oreochromis niloticus L. Aquaculture Research 30: 385–393.
Hargreaves JA (2013) Biofloc production systems for aquaculture; SRAC Publication No. 4503; Southern Regional Aquaculture Center Publication, Stoneville, MS, USA. pp. 1–12.
Jamal MT, Broom M, Al-Mur BA, Al Harbi M, Ghandourah M, ... Haque MF (2020) Biofloc technology: emerging microbial biotechnology for the improvement of aquaculture productivity. Polish Journal of Microbiology 69: 401–409.
Johari A, Ahmad AH, Jong CH, Kok ML, Kamaruddin MJ (2024) Analysis of biological factors on affecting the growth performance and water quality of biofloc aquaculture system for freshwater barramundi. E3S Web of Conferences, EDP Sciences, 04002.
Khalafalla MM, Ibrahim SA, Zayed MM, Awad MN, Mohamed RA (2020) Effect of a dietary mixture of beneficial bacteria on growth performance, health condition, chemical composition, and water quality of Nile tilapia, Oreochromis niloticus fingerlings. Journal of Aquatic Food Product Technology 29: 823–835.
Khanjani MH (2025) Evaluating the performance of Pacific white shrimp (Penaeus vannamei Boone, 1931) reared under different stocking densities: a study of their biochemical, immune, metabolic and antioxidant responses in a biofloc aquaculture system. Annals of Animal Science, DOI: 10.2478/aoas-2025-0087.
Khanjani MH, Mohammadi A, Emerenciano MGC (2024b) Water quality in biofloc technology (BFT): an applied review for an evolving aquaculture. Aquaculture International 32: 9321–9374.
Khanjani MH, Mozanzadeh MT, Sharifinia M, Emerenciano MGC (2024a) Broodstock and seed production in biofloc technology (BFT): an updated review focused on fish and penaeid shrimp. Aquaculture 579: 740278.
Khanjani MH, Sharifinia M, Hajirezaee S (2022) Recent progress towards the application of biofloc technology for tilapia farming. Aquaculture 552: 738021.
Khattab YA, Abdel-Tawwab M, Ahmad MH (2001) Effect of protein level and stocking density on growth performance, survival rate, feed utilization and body composition of Nile tilapia fry (Oreochromis niloticus L.). Egyptian Journal of Aquatic Biology and Fisheries 5(3): 195–212.
Lara-Flores M, Olivera-Castillo L, Olvera-Novoa MA (2010) Effect of the inclusion of a bacterial mix (Streptococcus faecium and Lactobacillus acidophilus), and the yeast (Saccharomyces cerevisiae) on growth, feed utilization and intestinal enzymatic activity of Nile tilapia (Oreochromis niloticus). International Journal of Fisheries and Aquaculture 2: 93–101.
Linh NV, Lubis AR, Dinh-Hung N, Wannavijit S, Montha N, ... Paolucci M (2024) Effects of shrimp shell-derived chitosan on growth, immunity, intestinal morphology, and gene expression of Nile tilapia (Oreochromis niloticus) reared in a biofloc system. Marine Drugs 22: 150.
Luczkovich JJ, Sprague MW, Paerl HW (2024) Bottom water hypoxia suppresses fish chorusing in estuaries. The Journal of the Acoustical Society of America 155: 2014–2024.
Mabroke RS, Tahoun AM, El-Haroun ER, Suloma A (2013) Influence of dietary protein on growth, reproduction, seed chemical composition and larval survival rate of Nile tilapia (Oreochromis niloticus) broodstocks of different size groups under hapa-in-pond hatchery system. Journal of Arabian Aquaculture Society 2: 203–220.
Mansour AT, Ashry OA, Ashour M, Alsaqufi AS, Ramadan KM, Sharawy ZZ (2022) The optimization of dietary protein level and carbon sources on biofloc nutritive values, bacterial abundance, and growth performances of whiteleg shrimp (Litopenaeus vannamei) juveniles. Life 12: 888.
Marty R (2003) Feeding tilapia in intensive recalculating system. United State, Department of Agriculture. Agricultural Research Service, Michigan State University, East Lansing. North Central Regional Aquaculture Center. pp. 1–4.
Mechaly AS, Awruch C, Cabrita E, Costas B, Fernandes JM, ... Ramos-Júdez S (2024) Cutting-edge methods in teleost and chondrichthyan reproductive biology. Reviews in Fisheries Science & Aquaculture 33(1): 77–112.
Meurer F, Novodworski J, Bombardelli RA (2024) Protein requirements in Nile tilapia (Oreochromis niloticus) during production and reproduction phases. Aquaculture and Fisheries 10(2): 171–182.
Ochieng Ogello E, Musa SM, Mulanda Aura C, Abwao JO, Mbonge Munguti J (2014) An appraisal of the feasibility of tilapia production in ponds using biofloc technology: a review. International Journal of Aquatic Science 5: 21–39.
Ribeiro MJP, Evangelista MM, Antônio E, Goncalves GS, Romagosa E (2017) Crude protein in diets for Nile tilapia broodfish. Boletim do instituto de Pesca 43: 35–46.
Santiago CB, Laron MA (2002) Growth and fry production of Nile tilapia, Oreochromis niloticus (L.), on different feeding schedules. Aquaculture Research 33: 129–136.
Shourbela RM, Khatab SA, Hassan MM, Van Doan H, Dawood MA (2021) The effect of stocking density and carbon sources on the oxidative status, and nonspecific immunity of Nile tilapia (Oreochromis niloticus) reared under biofloc conditions. Animals 11: 184.
Siddiqui A, Al‐Hafedh Y, Ali S (1998) Effect of dietary protein level on the reproductive performance of Nile tilapia, Oreochromis niloticus (L.). Aquaculture Research 29: 349–358.
Soliman AM, Abdel-Tawwab M (2022) Effects of different carbon sources on water quality, biofloc quality, and the productivity of Nile tilapia reared in biofloc-based ponds. Annals of Animal Science 22: 1281–1289.
Sousa SMN, Freccia A, Santos LD, Meurer F, Tessaro L, Bombardelli RA (2013) Growth of Nile tilapia post-larvae from broodstock fed diet with different levels of digestible protein and digestible energy. Revista Brasileira de Zootecnia 42: 535–540.
Teodósio R, Engrola S, Colen R, Masagounder K, Aragão C (2020) Optimizing diets to decrease environmental impact of Nile tilapia (Oreochromis niloticus) production. Aquaculture Nutrition 26: 422–431.
Turkmen S, Zamorano MJ, Fernández-Palacios H, Hernández-Cruz CM, Montero D, ... Izquierdo M (2017) Parental nutritional programming and a reminder during juvenile stage affect growth, lipid metabolism and utilisation in later developmental stages of a marine teleost, the gilthead sea bream (Sparus aurata). British Journal of Nutrition 118: 500–512.
Watanabe T, Arakawa T, Kitajima C, Fujita S (1984) Effect of nutritional quality of broodstock diets on reproduction of red sea bream. Nippon Suisan Gakkaishi 50: 495–501.
Xu WJ, Pan LQ (2014) Dietary protein level and C/N ratio manipulation in zero‐exchange culture of Litopenaeus vannamei: Evaluation of inorganic nitrogen control, biofloc composition and shrimp performance. Aquaculture Research 45: 1842–1851.
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