Anaesthetic effects of different concentrations of sodium bicarbonate on common carp (Cyprinus carpio)

Leah Joyce B. Gajutos; Aurelia B. Gajutos

doi:10.17017/j.fish.425

Leah Joyce B. Gajutos College of Arts and Sciences, Central Bicol State University of Agriculture, Calabanga Camarines Sur, 4405, Philippines
Aurelia B. Gajutos College of Veterinary Medicine, University of Eastern Philippines, University Town Catarman Northern Samar, 6400, Philippines

DOI: https://doi.org/10.17017/j.fish.425

Keywords: baking soda, common carp, Cyprinus carpio, dish anaesthetic, sodium bicarbonate

Abstract

Sedation of fish before handling and transporting can help curtail undue stress that can affect their health. This study investigated the effectiveness of sodium bicarbonate (NaHCO₃) utilised as an anaesthetic agent for common carp (Cyprinus carpio) with an average weight of 13.5 ± 0.55 g. The experimental fish were grouped and exposed to different concentrations at 0, 40, 45 and 50 g L^–1 of NaHCO₃ dissolved in water. Fishes were bath-immersed in different concentrations of NaHCO₃ solution until such time that they became anesthetised. Results showed that induction time and recovery time increases with increasing NaHCO₃ concentration. All fish reached complete anaesthesia in more than 3 minutes under a higher concentration of 50 g L^–1. No mortality occurred under any of the concentrations tested. The most optimal concentration leading to complete anaesthesia in 2 minutes and recovery in less than 4 minutes was 45 g L^–1. This study showed that NaHCO₃ is a potential fish anaesthetic for the common carp.

References

Altun T, Bilgin R, Danabas D (2009) Effects of sodium bicarbonate on anaesthesia of common carp (Cyprinus carpio L., 1758) juveniles. Turkish Journal of Fisheries and Aquatic Sciences 9: 29–31.

Avillanosa A, Marlowe C, Caipang A (2019) Use of sodium bicarbonate as an inexpensive general anesthetic for juvenile red tilapia hybrids. International Aquatic Research 11: 287–294.

Bolasina SN, de Azevedo A, Petry AC (2017) Comparative eﬃcacy of benzocaine, tricaine methanesulfonate and eugenolas anesthetic agents in the guppy Poecilia vivipara. Aquaculture Reports 6: 56–60.

Bowser PR (2001) Anesthetic options for fish. In: Gleed RD, Ludders JW (Eds) Recent advances in veterinary anaesthesia and analgesia: companion animals, international veterinary information service. Ithaca, New York, USA.

Coyle SD, Durborow M, Tidwell JH (2004) Anesthetic in aquaculture. Southern Regional Aquaculture Center (SRAC) Publication No. 3900.

FDA (2022) Animal veterinary. Aquaculture. Approved Aquaculture Drugs. U.S. Food and Drug Administration.

Fernandes IM, Bastos YF, Barreto DS, Lourenco LS, Penha JM (2017) The efficacy of clove oil as an anaesthetic and in euthanasia procedure for small-sized tropical fishes. Brazilian Journal of Biology 7(3): 444–450.

Freshwater Aquaculture (2019) Water quality in aquaculture. Freshwater Aquaculture. Retrieved July 10, 2022.

Githukia CM, Kembenyal EM, Opiyo MA (2016) Anaesthetic effects of sodium bicarbonate at different concentrations on African catfish (Clarias gariepinus) juveniles. Journal of Aquaculture Engineering and Fisheries Research 2(3): 151–158.

Hasimuna, OJ, Monde C, Mweemba M, Nsonga A (2019) The anaesthetic effects of sodium bicarbonate (baking soda) on greenhead tilapia (Oreochromis macrochir, Boulenger 1912) broodstock. The Egyptian Journal of Aquatic Research 46(2): 195–199.

Hseu, JR, Yeh SL, Chu YT, Ting YY (1998) Comparison of efficacy of five anaesthetic in goldlined sea bream, Sparus sarba. Acta Zoologica Taiwanica 9(1): 11–18.

Iversen M, Finstad B, McKinley RS, Eliassen RA (2003) The efficacy of metomidate, clove oil, Aqui-S™ and Benzoak® as anaesthetics in Atlantic salmon (Salmo salar L.) smolts, and their potential stress-reducing capacity. Aquaculture 221: 549–566.

Iwama GK, McGeer JC, Pawluk MP (1989) The effects of five fish anaesthetic on acid-base balance, hematocrit, cortisol and adrenaline in rainbow trout, Oncorhynchus mykiss. Canadian Journal of Zoology 67: 2065–2073.

Keene JL, Noakes DLG, Moccia RD, Soto CG (1998) The efficacy of clove oil as an anaesthetic for rainbow trout, Oncorhynchus mykiss (Walbaum). Canada Aquaculture Research 29: 89–101.

King WV, Hooper B, Hillsgrove S, Benton C, Berlinsk D (2005) The use of clove oil, metomidate, tricaine methanesulphonate and PE for inducing anaesthesia and their effect on the cortisol stress response in black sea bass, Centropristis striata. Aquaculture Research 36(14): 1442–1449.

Kristan J, Stara A, Polgesek M, Drasovean A, Kolarova J, Priborsky J (2014) Efficacy of different anaesthetics for pikeperch (Sander lucioperca L.) in relation to water temperature. Neuro endocrinology letters 35: 81–85.

Marking LL, Mayer FP (1985) Are better anaesthetics needed in fisheries? Fisheries 10 (6): 1–5.

Masoumeh E, Masoumeh B (2017) Effects of four anesthetics, clove extract, thyme extract, lidocaine, and sodium bicarbonate on the blood parameters and cortisol amount in grass carp (Ctenopharyngodon Idella). Journal of Marine Biology and Aquaculture 4(1): 1–4.

Masoumeh E, Masoumeh B (2018) Effects of four anesthetics, clove extract, thyme extract, lidocaine, and sodium bicarbonate on the blood parameters and cortisol amount in grass carp (Ctenopharyngodon idella). Journal of Marine Biology and Aquaculture 4(1): 1–4.

Mylonas CC, Cardinaletti G, Sigelaki I (2005) A comparative efﬁcacy of clove oil and 2-phenoxyethanol as anesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures. Aquaculture 246: 467–481.

Opiyo MA, Ogello EO, Charo Karisa H (2013) Effectiveness of sodium bicarbonate as an anaesthetic for different sizes of Nile tilapia, Oreochromis niloticus juveniles. International Journal of Aquatic Science 4(2): 14–22.

Palic D, Herolt DM, Andreason CB, Menzel BW, Roth JW (2006) Anaesthetic efficacy of tricaine methanesulfonate, metomidate and eugenol: effects on plasma cortisol concentration and neutrophil function in fathead minnows, Pimephales promelas. Aquaculture 254: 675–685.

Park IS (2019) The anesthetic effects of clove oil and MS-222 on far eastern catfish, Silurus asotus. Development and Reproduction 23(2): 183–191.

Park JY, Kim KR, Bang IC (2019) The anaesthetic effects of MS-222 for longthooh grouper, Epinephelus moara and hybrid grouper, E. moara ♀ × E. lanceolatus ♂. Korean Journal of Ichthyology 31: 235–240.

Popovic NT, Strunjak-Perovic I, Coz-Rakovac R, Barisic J, Jadan M, ... Sauerborn Klobucar R (2012) Tricaine methane-sulfonate (MS-222) application in fish anesthesia. Journal of Applied Ichthyology 28(4): 553–564.

Ramlochansingh C, Brononer F, Chagnaud BP, Straka H (2014) Efficacy of tricaine methanesulfonate (MS-222) as an anesthetic agent for blocking sensory-motor responses in Xenopus laevis tadpoles. PLoS ONE 9(7): e101606.

Ross LG, Ross B (2008) Anaesthetic and sedative techniques for aquatic animals. Blackwell, Oxford, UK.

Ross, LG, Ross B (1999) Anaesthetic and sedative techniques for aquatic animals. Blackwell Science, Oxford.

Shokr EAM, Fathy A (2018) Effect of quinaldine sulfate, clove oil and tricaine methanesulfonate anesthesia on physiological, biochemical parameters and mortality of Nile tilapia (Oreochromis niloticus). Abbassa International Journal of Aquaculture 11(2): 223–240.

Skar MW, Haugland GT, Powell MD, Wergeland HI, Samuelsen OB (2017) Development of anesthetic protocols for lumpfish (Cyclopterus lumpus L.): effect of anaesthetic concentrations, sea water temperature and body weight. PLoS One 12(7): e0179344.

Tsantilas H, Galatos AD, Athanassopoulou F, Prassinos NN, Kousoulaki K (2006) Efficacy of 2-phenoxyethanol as an anaesthetic for two size classes of white sea bream, Diplodus sargus L., and sharp snout sea bream, Diplodus puntazzo C. Aquaculture 253: 64–70.

Varkey AMT, Sajeevan S (2014) Efficacy of 2-phenoxyethanol as an anaesthetic for adult redline torpedo fish, Sahyadria denisonii (Day 1865). International Journal of Zoology 2014: 315029.

Velisek J, Wlasow T, Gomulka P, Svobodova Z, Novotny (2007) Effects of 2-phenoxy ethanol anaesthesia on sheatfish (Silurus glanis L.). Veterinarni Medicina 52(3): 103–110.

Yildiz M, Kayim M, Akin S (2013) The anesthetic effects of clove oil and 2-phonoxyethanol on rainbow trout, Oncorhynchus mykiss at different concentrations and temperatures. Iranian Journal of Fisheries Sciences 12: 947–961.

Zahl IH, Kiessling A, Samuelsen OB, Olsen RE (2010) Anesthesia induces stress in Atlantic salmon (Salmo salar), Atlantic cod (Gadus morhua) and Atlantic halibut (Hippoglossus hippoglossus). Fish Physiological Biochemistry 36: 719–730.

Zaikov A, Iliev I, Hubenova T (2008) Induction and recovery from anesthesia in pike, Esox lucius exposed to clove oil. Bulgarian Journal of Agriculture Science 14: 165–170.