Evaluation of the reproductive cycle and gonadal development in the climbing perch, Anabas testudineus (Bloch, 1792) in captivity

Chokki Veettil Priyatha; Kumari Chidambaran Chitra

doi:10.17017/j.fish.364

Chokki Veettil Priyatha Endocrinology and Toxicology Laboratory, Department of Zoology, University of Calicut, Malappuram District, Kerala 673635, India
Kumari Chidambaran Chitra Endocrinology and Toxicology Laboratory, Department of Zoology, University of Calicut, Malappuram District, Kerala 673635, India

DOI: https://doi.org/10.17017/j.fish.364

Keywords: Anabas testudineus, gonadosomatic index, hepatosomatic index, histology, serum sex hormones

Abstract

The climbing perch Anabas testudineus, the most demanded indigenous fish species of India, were used for the evaluation of reproductive strategy and gonadal development at first maturation under the controlled laboratory condition. In both sexes, four distinct gonadal developmental stages namely preparatory, pre-spawning, spawning, and post-spawning were identified based on the gonadosomatic index (GSI), hepatosomatic index (HSI), gonadal morphology, and the levels of serum sex hormones. The spawning stage was identified from July and August and the fecundity ranged from 6500–11000 eggs. The GSI exhibited a significant increase from preparatory to pre-spawning and spawning stages, and maintained high until August. The HSI was maintained the same during preparatory, pre-spawning, and spawning stages while increased in post-spawning stage in both sexes. Serum estrogen and testosterone levels increased significantly during pre-spawning, and spawning stages in both sexes. Fecundity showed a positive correlation with the length, weight, and ovary weight. Histological sections showed that male A. testudineus were group-synchronous spawners. Both sexes showed discrete reproductive cycles characterized by distinct changes in gonadal size, sex hormones and gonadal indices. This study will bridge the gap of insufficient knowledge to support the best practices suitable for the A. testudineus culture and survival under captivity.

References

Amornsakun T, Sriwatana W, Promkaew P (2005) Some aspects in early life stage of climbing perch, Anabas testudineus larvae. Journal of Science and Technology 27(1): 403–418.

APHA (1998) Standard methods for the examination of water and wastewater. American Public Health Association, American Water Works Association and Water Environment Federation. Washington, D.C.

Ara I, Nabi MR (2018) Morphometric and meristic comparison of local and Thai Anabas testudineus. Journal of Fisheries 6(2): 599–604.

Araujo FG, Nascimento AA, Gomes ID, Sales A, Chagas de Oliveira BA (2019) Gonadal development and reproductive period of the characin Astyanax aff. bimaculatus (Characiformes: Characidae) in a tropical reservoir in southeastern Brazil. Zoologia 36: e30610.

Banu N, Ali S, Saha T, Vakta N (1985) The studies on the fecundity of Anabas testudineus (Bloch) in a confined pond of Dhaka district. Bangladesh Journal of Aquaculture 6(7): 45–49.

Behera S, Devi LM, Kumar S, Gogoi R, Samanta P, Jomang O, Baksi S (2015) External morphology and sexual dimorphism of Anabas testudineus in natural environment. International Journal of Science and Nature 6(2): 288–292.

Bernal RAD, Aya FA, De Jesus-Ayson EGT, Garcia LMB (2015) Seasonal gonad cycle of the climbing perch Anabas testudineus (Teleostei: Anabantidae) in a tropical wetland. Ichthyology Research 62(4): 389–395.

Bhattacharjee I, Chandra G (2016) Food and feeding habits of three air-breathing fish in its natural habitat. International Journal of Fisheries and Aquatic Studies 4(3): 586–589.

Bhattacharyya M, Homechaudhuri S (2009) Assessment of captive breeding of Anabas testudineus with the synthetic hormone, ovaprim. Proceedings of the Zoological Society 62: 23–27.

Blazer VS (2002) Histopathological assessment of gonadal tissue in wild fishes. Journal of Physiology and Biochemistry 26: 85–101.

Bromage N, Jones J, Randall C, Thrush M, Davies B, ... Barker G (1992) Broodstock management, fecundity, egg quality and the timing of egg production in the rainbow trout (Oncorhynchus mykiss). Aquaculture 100(1-3): 141–166.

Chakraborti P, Bhattacharya S (1984) Plasma thyroxine levels in freshwater perch: influence of season, gonadotropins, and gonadal hormones. General and Comparative Endocrinology 53(2): 179–186.

Chaube R, Mishra S, Singh RK (2018) A comparison of steroid profiles in the testis and seminal vesicle of the catfish (Heteropneustes fossilis). Theriogenology 105: 90–96.

Chellappa S, Huntingford F, Strang R, Thomson R (1995) Condition factor and hepatosomatic index as estimates of energy status in male three‐spined stickleback. Journal of Fish Biology 47: 775–787.

Coward K, Bromage NR (1999) Spawning periodicity, fecundity and egg size in laboratory-held stocks of a substrate-spawning tilapiine, Tilapia zillii (Gervais). Aquaculture 171(3-4): 251–267.

Dehadrai P, Banerji S, Thakur N, Das N (1973) Sexual dimorphism in certain air breathing teleosts. Journal of the Inland Fisheries Society of India 5(7): 1–77.

Froese R, Pauly D (2014) FishBase, World Wide Web electronic publication. http://www.fishbase.org

Galib SM, Samad MA, Hossain MA, Mohsin ABM, Haque SMM (2010) Small Indigenous Species of Fishes (SISF) in Chalan Beel with reference to their harvesting and marketing. Bangladesh Journal of Progressive Science and Technology 8(2): 251–254.

Grimes CB, Huntsman GR (1980) Reproductive biology of vermilion snapper, Rhomboplites aurorubens from North Carolina and South Carolia. Fisheries Bulletin 78: 137–146.

Hismayasari IB, Marhendra APW, Rahayu S, Saidin SD, Supriyadi D (2015) gonadosomatic index (GSI), hepatosomatic index (HSI) and proportion of oocytes stadia as an indicator of rainbowfish Melanotaenia boesemani spawning season. International Journal of Fisheries and Aquatic Studies 2: 359–362.

IUCN (2019) IUCN Red list of threatened species, version 2014.2. https://www.iucnredlist.org. Accessed on 12 January 2022.

Jacob PK (2005) Studies on some aspects of reproduction of female Anabas testudineus (Bloch). Doctoral Thesis, Cochin University of Science and Technology, India.

Jakobsen T, Fogarty MJ, Megrey BA, Moksness E (2009) Fish reproductive biology: implications for assessment and management. Blackwell, Oxford.

Jan M, Ahmed I (2016) Assessment of fecundity, gonadosomatic index and hepatosomatic index of snow trout, Schizothorax plagiostomus in river Lidder, from Kashmir Himalaya, India. International Journal of Fisheries and Aquatic Studies 14(2): 370–375

Juntti SA, Fernald RD (2016) Timing reproduction in teleost fish: cues and mechanisms. Current Opinion in Neurobiology 38: 57–62.

King M (1995) Fisheries biology assessment and management. Fishing News Books Blackwell Science Ltd., London. 341 pp.

Koc ND, Aytekin Y, Yuce R (2008) Ovary maturatıon stages and histological investigation of ovary of the zebrafish (Danio rerio). Brazilian Archives of Biology and Technology 51(3): 513–522.

Lagler KF, Bardach J, Miller R, Passino D (1977) Ichthyology. John Willey and Sons Inc., New York.

Larson GL (1973) Liver weight of brook trout in a high-mountain lake in Washington State. The Progressive Fish-Culturist 35(4): 234–236.

Lawson EO (2011) Length-weight relationships and fecundity estimates in mudskipper, Periophthalmus papilio (Bloch and Schneider, 1801) caught from the mangrove swamps of Lagos Lagoon, Nigeria. Journal of Fisheries and Aquatic Sciences 6: 264–271.

Mahboob S, Sheri A (2002) Relationships among gonad weight, liver weight and body weight of major, common and some Chinese carps under composite culture system with special reference to pond fertilization. Asian-Australasian Journal of Animal Sciences 15(5): 740–744.

Marimuthu K, Arumugam J, Sandragasan D, Jegathambigai R (2009) Studies on the fecundity of native fish climbing perch (Anabas testudineus, Bloch) in Malaysia. American-Eurasian Journal of Sustainable Agriculture 3(3): 266–274.

Meethal SV, Atwood CS (2005) The role of hypothalamic-pituitary-gonadal hormones in the normal structure and functioning of the brain. Cellular and Molecular Life Sciences 62(3): 257–270.

Miranda ACL, Bazzoli N, Rizzo E, Sato Y (1999) Ovarian follicular atresia in two teleost species: a histological and ultrastructural study. Tissue and Cell 31: 480–488.

Montchowui E, Compère P, Thiry M, Laleye P, Philippart JC, Poncin P (2012) Histological assessment of gonad maturation in Labeo parvus (Teleostei: Cyprinidae) in Benin. The African Journal of Aquatic Science 37(2): 155–163.

Morioka S, Ito S, Kitamura S, Vongvichith B (2009) Growth and morphological development of laboratory-reared larval and juvenile climbing perch Anabas testudineus. Ichthyological Research 56: 162.

Nagahama Y (1994) Endocrine regulation of gametogenesis in fish. International Journal of Developmental Biology 38: 217–229.

Nagahama Y, Yamashita M (2008) Regulation of oocyte maturation in fish. Development Growth and Differentiation 50: S195–S219.

Ndobe S, Masyahoro A, Serdiati N, Moore AM (2020) Reproductive and morphometric characteristics of climbing perch Anabas testudineus in Sigi, Central Sulawesi, Indonesia. AACL Bioflux 13: 167–182.

Newton SH, Kilambi RV (1969) Determination of sexual maturity of white bass from ovum diameters. The Southwestern Naturalist 14(2): 213–220.

Nishimura T, Yamada K, Fujimori C, Kikuchi M, Kawasaki T, ... Tanaka M (2018) Germ cells in the teleost fish medaka have an inherent feminizing effect. PLoS Genetics 14: 1–18.

OECD (2019) Test No. 203: fish, acute toxicity test, OECD guidelines for the testing of chemicals, section 2. OECD Publishing, Paris.

Pal P, Moulik SR, Gupta S, Guha P, Majumder S, ... Mukherjee D (2018) Estrogen‐regulated expression of P450arom genes in the brain and ovaries of adult female Indian climbing perch, Anabas testudineus. Journal of Experimental Zoology Part A Ecological and Integrative Physiology 329(1): 29–42.

Pandit DN, Gupta M (2019) Heapto-somatic index, gonado-somatic index and condition factor of Anabas testudineus as bio-monitoring tools of nickel and chromium toxicity. International Journal of Innovative Science Engineering and Technology 12(3): 25–28.

Patino R, Sullivan CV (2002) Ovarian follicle growth, maturation, and ovulation in teleost fish. Fish Physiology Biochemistry 26: 57–70.

Pelissero C, Flouriot G, Foucher JL, Bennetau B, Dunogues J, ... Sumpter JP (1993) Vitellogenin synthesis in cultured hepatocytes: an in vitro test for the estrogenic potency of chemicals. Journal of Steroid Biochemistry and Molecular Biology 44(3): 263–272.

Pham HQ, Le HM (2020) Seasonal changes in three indices of gonadal maturation in male golden rabbitfish (Siganus guttatus): implications for artificial propagation. Fish Physiology and Biochemistry 46(3): 1111–1120.

Pham HQ, Nguyen AT, Kjorsvik E, Nguyen MD, Arukwe A (2012) Seasonal reproductive cycle of Waigieu seaperch (Psammoperca waigiensis). Aquaculture Research 43: 815–830.

Rajbongshi A, Ali A, Chakravarty M, Deka M, Mazumdar H, ... Baishya S (2020) Efficiency of ovatide on mass seed production of climbing perch (Anabas testudineus, Bloch, 1972) in Nalbari district, Assam. The Bioscan 15(3): 323–326.

Redding MJ, Patino R (1993) Reproductive physiology. In: Evans DH (Ed) The physiology of fishes (pp. 503-534). Marine Science Series. CRC, Boca Raton, FL, USA.

Roberts RJ, Smail DA (2001) Laboratory methods. In: Roberts RJ (Ed) Fish pathology, 3rd edition. Harcourt Publishers Limited, USA. pp. 380–390.

Rutaisire J, Muwazi RT, Booth AJ (2003) Structure and cytology of the testes of Labeo victorianus (Pisces: Cyprinidae). African Zoology 38(1): 119–126.

Sajan S, Mercy TA (2016) Characterization of testis and sperm morphology of Sahyadria denisonii (Day 1865) an endemic and threatened ornamental fish of the Western Ghats of India. Turkish Journal of Fisheries and Aquatic Sciences 16(3): 531–538.

Samad MA, Asaduzzaman M, Galib SM, Kamal MM, Haque MR (2010) Availability and consumer preference of small indigenous species (SIS) of the River Padma at Rajshahi, Bangladesh. International Journal of BioResearch 1(5): 27–31.

Schulz RW, de França LR, Lareyre JJ, LeGac F, Chiarini-Garcia H, ... Miura T (2010) Spermatogenesis in fish. General and Comparative Endocrinology 165(3): 390–411

Selman K, Wallace RA (1989) Cellular aspects of oocyte growth in teleosts. Zoological Science 6: 211–231.

Singh S, Srivastava A (2015) Variations in hepatosomatic index (HSI) and gonadosomatic index (GSI) in fish Heteropneustes fossilis exposed to higher sub-lethal concentration to arsenic and copper. Journal of Ecophysiology and Occupational Health 15(3/4): 89.

Sulistyo I, Fontaine P, Rincarh J, Gardeur JN, Migaud H, ... Kestemont P (2000) Reproductive cycle and plasma level of steroid in male Eurasian perch (Perca fluviatilis). Aquatic Living Resources 13: 99–106.

Taranger GL, Carrillo M, Schulz RW, Fontaine P, Zanuy S, ... Hansen T (2010) Control of puberty in farmed fish. General and Comparative Endocrinology 165: 483–515.

Uribe MC, Grier HJ, Mejía-Roa V (2015) Comparative testicular structure and spermatogenesis in bony fishes. Spermatogenesis 4(3): e983400.

Wang W, Zhu H, Dong Y, Tian Z, Dong T, ... Niu C (2017) Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species. Fish Physiology and Biochemistry 43(6): 1557–1569.

Yaron Z, Levavi-Sivan B (2011) Endocrine regulation of fish reproduction. In: Farrell AP (Ed) Encyclopedia of fish physiology: from genome to environment. Academic Press, San Diego. pp. 1500–1508.

Ziauddin G, Behera S, Kumar S, Gogoi R, Jomang O, Baksi S (2016) Morphometrical and gonadal studies of a threatened fish, Anabas testudineus with respect to seasonal cycle. International Journal of Fisheries and Aquatic Studies 6(1): 7–14.

Zworykin D (2012) Reproduction and spawning behavior of the climbing perch Anabas testudineus (Perciformes, Anabantidae) in an aquarium. Journal of Ichthyology 52: 379–388.