Are haematological and biochemical properties of freshwater spotted snakehead Channa punctata (Bloch, 1793) season and sex dependent?

  • Minakshi Singh Fish Biology Research Lab, Department of Zoology, University of Lucknow, Lucknow (U.P.), India
  • Ankur Kashyap Fish Biology Research Lab, Department of Zoology, University of Lucknow, Lucknow (U.P.), India; Junior High School, Govindpur, Mohanlalganj, Lucknow (U.P.), India
  • Mohammad Serajuddin Fish Biology Research Lab, Department of Zoology, University of Lucknow, Lucknow (U.P.), India
Keywords: Channa punctatus, fish health, murrel, physiology, fish physiology


Freshwater murrel, Channa punctata commonly known as “spotted snakehead” is a popular fish because of its taste and price. Haematological and biochemical profile of fish helps in understanding the fish health and play an important role in fisheries management. The present work was carried out to determine season (summer, monsoon and winter) and sex dependent variations in the blood profile of C. punctata. Haematological [red blood cell counts (RBC), packed cell volume (PCV) and haemoglobin (Hb)] and serum biochemical parameters [blood glucose, total protein, cholesterol, triglyceride (Tg), low density lipoprotein (LDL), very low density lipoprotein (VLDL), high density lipoprotein (HDL), serum calcium levels] of C. punctata were analysed which varied significantly with seasons (p < 0.05). The level of glucose, total protein, Tg, VLDL, RBC, Hb and PCV was low during monsoon season because of the growth and development of gonads compared to the summer and winter season. The level of cholesterol and LDL was high during monsoon. However, haematological and biochemical parameters were not sex dependent. The seasonal haematological and biochemical variations in the fish were mainly due to the physiological acclimatisation of the fish to variations in the environmental condition and feeding regime.


Abdel‐Hameid NAH, Zehra S, Khan MA (2017) Dietary copper requirement of fingerling Channa punctatus (Bloch) based on growth, feed conversion, blood parameters and whole body copper concentration. Aquaculture Research 48(6): 2787–2797.

Acharya G, Mohanty PK (2014) Haematological and serum biochemical parameters in different sexes of walking cat fish, Clarias batrachus (Linnaeus, 1758). International Journal of Science and Research 3(8): 1914–1917.

Alyakrinskyaya IO, Dolgova SN (1984) Hematological features of young sturgeons. Vopr Ikhtiol 4: 135–139.

Babin PJ, Vernier JM (1989) Plasma lipoproteins in fish. Journal of Lipid Research 30(4): 467–489.

Bani A, Vayghan AH (2011) Temporal variations in haematological and biochemical indices of the Caspian kutum, Rutilus frisii kutum. Ichthyological Research 58(2): 126–133.

Bhaskar BR, Rao KS (1990) Use of haematological parameters as diagnostic tools in determining the health of milkfish, Chanos chanos (Forskal), in brackishwater culture. Aquaculture Research 21(1): 125–129.

Chaki N, Jahan S, Fahad MFH, Galib SM, Mohsin ABM (2014) Environment and fish fauna of the Atrai River: global and local conservation perspective. Journal of Fisheries 2(3): 163-172.

Dacie JV, Lewis SM (1984) Practical hematology. Churchill Livingstone, Edinburgh.

Ebeh HN, Ogbonna SA, Didigwu Nwani C (2017) Plasma biochemistry, haematological and biometric reference values of Protopterus annectens of Anambra River, Nigeria. Zoology and Ecology 27(1): 74–81.

Fallah FJ, Khara H, Roohi JD, Boorani MS (2014) Hematological parameters of pike Esox lucius in relation to different ages and seasons. Comparative Clinical Pathology 23(4): 949–953.

Fazio F, Saoca C, Piccione G, Kesbiç OS, Acar Ü (2016) Comparative study of some hematological and biochemical parameters of Italian and Turkish farmed rainbow trout Oncorhynchus mykiss (Walbaum, 1792). Turkish Journal of Fisheries and Aquatic Sciences 16(3): 715–721.

Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical chemistry 18(6): 499–502.

Froese R, Pauly D (2019) FishBase. World Wide Web electronic publication., version (12/2019).

Galib SM, Mohsin ABM, Parvez MT, Lucas MC, Chaki N, ... Islam MN (2018) Municipal wastewater can result in a dramatic decline in freshwater fishes: a lesson from a developing country. Knowledge and Management of Aquatic Ecosystems 419, 37.

Galib SM, Rashid MA, Chaki N, Mohsin ABM, Joadder MAR (2016) Seasonal variation and community structure of fishes in the Mahananda River with special reference to conservation issues. Journal of Fisheries 4(1): 325-334.

Galib SM, Samad MA (2009) Harvesting, traditional preservation and marketing of fishes of Chalan Beel, Bangladesh. Aquaculture Asia XIV(1): 12-15

Galib SM, Samad MA, Hossain MA, Mohsin ABM, Haque SMM (2010) Small Indigenous Species of Fishes (SISF) in Chalan Beel with reference to their harvesting and marketing. Bangladesh Journal of Progressive Science and Technology 8(2): 251–254.

Ghosh S (2006) Composition and distribution of lipids and fatty acids in two jeol fishes of West Bengal. PhD thesis, University of Calcutta, Calcutta, India.

Gutiérrez J, Fernandez J, Planas J (1988) Seasonal variations of insulin and some metabolites in dogfish plasma, Scyliorhinus canicula, L. General and Comparative Endocrinology 70(1): 1–8.

Hutchinson TH, Manning MJ (1996) Seasonal trends in serum lysozyme activity and total protein concentration in dab (Limanda limanda L.) sampled from Lyme Bay, UK. Fish & Shellfish Immunology 6(7): 473–482.

Imteazzaman AM, Galib SM (2013) Fish fauna of Halti Beel, Bangladesh. International Journal of Current Research 5(1): 187–190.

Javed M, Ahmad I, Ahmad A, Usmani N, Ahmad M (2016) Studies on the alterations in haematological indices, micronuclei induction and pathological marker enzyme activities in Channa punctatus (spotted snakehead) Perciformes, Channidae exposed to thermal power plant effluent. SpringerPlus 5(1): 761.

Javed M, Usmani N (2015) Stress response of biomolecules (carbohydrate, protein and lipid profiles) in fish Channa punctatus inhabiting river polluted by thermal power plant effluent. Saudi Journal of Biological Sciences 22(2): 237–242.

Kalsoom S, Nasreen Z (2019) Risk assessment of persistent organic pollutants in fishes from Pakistan and its neighbouring countries. Journal of Fisheries 7(3): 726–740.

Kashyap A (2015) Intraspecific diversity and reproductive biology in freshwater murrel, Channa punctatus (Bloch). PhD Dissertation, Department of Zoology, Lucknow University, Lucknow, India.

Kashyap A, Awasthi M, Serajuddin M (2016) Reproductive biology of the freshwater murrel Channa punctatus (Bloch, 1793) from River Gomti of Lucknow region, Uttar Pradesh, India. Indian Journal of Fisheries 63(3): 126–130.

Kocaman EM, Yanik T, Erdogan O, Ciltas AK (2005) Alteration in Cholesterol, glucose and triglyceride levels in reproduction of rainbow trout Oncorhynchus mykiss. Journal of Animal and Veterinary Advances 4(9): 801–804.

Latif M, Ali M, Iqbal F (2015) Seasonal variations in hematological and serum biochemical profile of Channa marulius are complementary to the changes in water quality parameters of river Chenab in Pakistan. Pakistan Journal of Zoology 47(6): 1699–1707.

Lermen CL, Lappe R, Crestani M, Vieira VP, Gioda CR, Schetinger MR, Baldisserotto B, Moraes G, Morsch VM (2004) Effect of different temperature regimes on metabolic and blood parameters of silver catfish Rhamdia quelen. Aquaculture 239(1–4):497–507.

Malathi K, Kannathasan A, Rajendran K (2012) Comparative haematological studies on fresh water fishes Channa punctatus and Channa striatus (BLOCH). International journal of Pharmaceutical, Chemical and Biological Sciences 2(4): 644–648.

Nasari FH, Kochanian P, Salati AP, Pashazanoosi H (2014) Variation of some biochemical parameters in female yellowfin seabream, Acanthopagrus latus (Houttuyn) during reproductive cycle. Folia Zoologica 63(4): 238–245.

Parveen S, Singh D, Bharose R, Rout S, Khan MA, Ansari EF (2017) Tannery effluent effect on the haematological parameters of freshwater fish, Channa punctatus. Journal of Applied and Natural Science 9(1): 201–205.

Percin F, Konyalioglu S (2008) Serum biochemical profiles of captive and wild northern bluefin tuna (Thunnus thynnus L. 1758) in the Eastern Mediterranean. Aquaculture Research 39(9): 945–953.

Pethiyagoda R (1991) Freshwater fishes of Sri Lanka. The Wildlife Heritage Trust of Sri Lanka, Colombo.

Pradhan SC, Patra AK, Pal A (2014) Hematological and plasma chemistry of Indian major carp, Labeo rohita (Hamilton, 1822). Journal of Applied Ichthyology 30(1): 48–54.

Rahman AKA (1989) Freshwater fishes of Bangladesh. Zoological Society of Bangladesh. Department of Zoology, University of Dhaka.

Rossi A, Bacchetta C, Cazenave J (2017) Effect of thermal stress on metabolic and oxidative stress biomarkers of Hoplosternum littorale (Teleostei, Callichthyidae). Ecological Indicators 79: 361–370.

Srivastav SK, Srivastav AK (1998) Annual changes in serum calcium and inorganic phosphate levels and correlation with gonadal status of a freshwater murrel, Channa punctatus (Bloch). Brazilian Journal of Medical and Biological Research 31(8): 1069–1073.

Svobodova Z, Kolarova J, Kouril J, Hamackova J, Vykusová B, Kaláb P (1997) Haematological investigations in Silurus glanis L. females during pre-and post-spawning period. Polish Archives of Hydrobiology 44(1–2): 67–81.

Talukdar B, Kalita HK, Baishya RA, Basumatary S, Dutta A, Srivastava SK, Sarma D (2017) Effects of acid mine drainage of coal mines on some haematological parameters of Channa punctatus (Bloch). National Academy Science Letters 40(2): 91–94.

Valverde JC, García BG (2005) Suitable dissolved oxygen levels for common octopus (Octopus vulgaris Cuvier, 1797) at different weights and temperatures: analysis of respiratory behaviour. Aquaculture 244(1–4): 303–314.

Zorriehzahra MJ, Hassan MD, Gholizadeh M, Saidi AA (2010) Study of some hematological and biochemical parameters of Rainbow trout (Oncorhynchus mykiss) fry in western part of Mazandaran province, Iran. Iranian Journal of Fisheries Sciences 9(1): 185–198.

How to Cite
Singh, M., Kashyap, A., & Serajuddin, M. (2020). Are haematological and biochemical properties of freshwater spotted snakehead Channa punctata (Bloch, 1793) season and sex dependent?. Journal of Fisheries, 8(3), 878-884. Retrieved from