Growth performance, haematology and histopathology of African catfish (Clarias gariepinus) fed varying levels of Aloe barbadensis leaves
Abstract
One hundred and twenty Clarias gariepinus fingerlings (2.33 ± 0.07 g) were fed with 40% crude protein diets containing three concentrations of Aloe barbadensis leaves-paste: ABL1, 1%; ABL2, 2%; ABL3, 3%, and control, 0% ad libitum twice daily for 12 weeks. Mean weight gain and percentage weight gain increased (P < 0.05) as concentration of A. barbadensis increased. Survival rate decreased as concentration of paste increased. Differences (P < 0.05) seen in packed cell volume (PCV), haemoglobin (Hb), and red blood cell (RBC), thus highest in ABL3: PCV (36.67 ± 0.89%), Hb (12.37 ± 0.37 g dl–1) and RBC (3.47 ± 0.08×106 L–1) and lowest in control: PCV (22.0 ± 0.58%), Hb (7.37 ± 0.20 g dl–1) and RBC (2.07 ± 0.06 ×106 L–1). Liver histology of control fish was normal, while fatty degenerations were seen in the treated fish. The histology of fish kidney was normal in all treatments. The study concluded that 1% A. barbadensis leaves-paste could effectively improve growth performance, nutrient utilization and survival of cultured C. gariepinus.
References
Abd-El-Rhman AMM (2009) Antagonism of Aeromonas hydrophila by propolis and its effect on the performance of Nile tilapia, Oreochromis niloticus. Fish & Shellfish Immunology 27(3): 454–459.
Abou-zeid SM (2002) The effect of some medical plant on reproductive and productive performance of Nile tilapia, Oreochronis niloticus. PhD Thesis, Faculty of Agriculture, Cairo University, Egypt. 212 pp.
Adedeji OS, Farimi GO, Ameen SA and Olayemi JB (2008) Effects of bitter cola (Garcinia kola) as growth promoter in Broiler Chicks from day old to four weeks old. Journal of Animal and Veterinary Advances 5(3): 191–193.
Agbebi OT, Ilesanmi AI and AbdulRaheem I (2013) Additive potentials of Z. officinalein the aflatoxin secreted in waste mould feed fed to Clarias gariepinus (Burchell, 1822). International Journal of Biological and Chemical Science 7(1): 236–246.
Agbebi OT, Lawal HB and Odebiyi VC (2012) Aflatoxin effect of moulded gel waste mixed with ginger and its histopathological study on Clarias garepinus. Global Journal of Science Frontier Research 12(1): 7–15.
Alicia ET, Magariños B and Romalde JL (2005) A review of the main bacterial fish diseases in mariculture system. Aquaculture 246(1–4): 37–61.
Alishahi M, Ranjbar MM, Ghorbanpour M, Peyghan R, Mesbah M and Razi Jalali M (2010) Effects of dietary Aloe vera on some specific and nonspecific immunity in the common carp (Cyprinus carpio). International Journal of Veterinary Research 4: 189–195.
AOAC (2011) Association of International Official Analytic Chemists, AOAC International, 18th edition, Arlington, Virginia.
Ashade OO, Adelusi OE and Ligali NA (2014) Histopathological effects of untreated ginger peel (Zingiber officinale) fish meal on the intestine tissue profiling of African catfish (Clarias gariepinus). International Journal of Fisheries and Aquatic Studies 2: 95–98.
Bamidele NA, Obasa SO, Ikeiwenwe NB, Abdulraheem I, Adeoye AA and Odebiyi OC (2015) Effect of dried moringa (Moringa oleifera) seed meal based diet on the growth, haematological, biochemical parameter and histopathology of African catfish, Clarias gariepinus fingerlings. International Journal of Fisheries and Aquatic Studies 2: 27–34.
Bawankar R, Singh P and Subramanaian B (2004) Bioactive compounds and medicinal properties of Aloe vera L.: an update. Journal of Plant Sciences 2(3): 102–107.
Bello OS, Emikpe BO and Olaifa FE (2012) The body weight changes and gut morphometry of Clarias gariepinus juveniles on feeds supplemented with walnut (Tetracarpidium conophorum) leaf and onion (Allium cepa) bub residues. International Journal of Morphology 30(1): 253–251.
Chandrakesan PK, Muralidharan VD, Kumar G, Ponnudurai TJ, Harikrishnan KS and Pani VN (2001) Efficacy of an herbal complex against caecal coccidiosis in broiler chickens. Veterinarski Archive 79: 199–203.
Choi S and Chung MH (2003) A review on the relationship between aloe vera components and their biological effects. Seminars in Integrative Medicines 1(1): 53–62.
Dacie JV and Lewis SM (2001) Practical Haematology 9th Edition, Churchill Livingstone, London. 633 pp.
Darabighane B, Abolfazl Z, Ahmad ZS and Akhtar (2008). Using aqeous extract of aloe gel as anticoccidial and immunostimulant agent in broiler production. Sarhad Journal of Agriculture 24: 665–669.
Daudo OM (2012) Effect of ginger by-product meal supplementation with enzyme/palm oil on performance, blood chemistry and histopathology of broiler chickens. PhD Thesis, Ahmadu Bello University, Zaria, Nigeria. 205 pp.
Davies OA, Inko-Tariah MB and Amachree D (2006) Growth response and survival of heterobronchus longitilus fingerlings fed at different feeding frequencies. African Journal of Biotechnology 5: 788–787.
De Pedro N, Guijarro AI, López-Patiño MA, Martínez-Álvarez R and Delgado MJ (2005) Daily and seasonal variations in haematological and blood biochemical parameters in the tench, Tinca tinca Linnaeus, 1758. Aquaculture Research 36: 1185–1196.
De Silva SS (2001) Performance of Oreochromis niloticus fry maintained on mixed feeding schedules of different protein levels. Aquaculture and Fisheries 16: 621–633.
Denev SA (2008) Ecological alternatives of antibiotic growth promoters in the animal’s husbandry and Aquaculture. DSc Thesis, Department of Biochemistry, Microbiology, Trakia University, Stara Zagora, Bulgaria. 294 pp.
Dienye HE and Olumuyi OK (2014) Growth performance and haematological responses of African mud catfish Clarias gariepinus fed dietary levels of Moringa oleifera leaf meal. Net Journal of Agricultural Science 2(2): 79–88.
Douglas JW and Jane KW (2001) In Schalm’s Veterinary Haematology. John Willey and Sons, Blackwell Publishing Ltd. 1232 pp.
Duncan DB (1955) Multiple ranges and multiple F-tests. Biometrics 11: 1–42.
Ezeri GNO (2001) Haematological responses of Clarias gariepinus to bacterial infection and prophylactic treatment with antibiotics. Journal of Aquatic Science 16: 22–24.
Farah A, Kasiri M, Sudager M, Soleiman IM and Zorrieh Zahra SMJ (2012) Effect of dietary supplementation of Mellisa officinalis and Aloe vera on haematological traits, lipid oxidation of carcass and performance in rainbow trout (Oncorhynchus mykiss). Online Journal of Animal and Feed Research 2(1): 1–5.
FAO (2016) State of world fisheries and aquaculture, contributing to food security and nutrition for all. Food and Agriculture Organization, Rome.
Gabriel UU, Alagon JK and Allison ME (2001) Effects of dispersed crude oil water dispersion on the haemoglobin and haematocrit of Clarias gariepinus. Journal of Aquatic Science Environmental Management 5(2): 9–11.
Galib SM, Mohsin ABM, Chaki N, Fahad MFH, Haque SMM (2013) An overview of the traditional rice-prawn-fish farming in Kalia of Narail District, Bangladesh. Journal of Fisheries 1(1): 1–6. doi: 10.17017/jfish.v1i1.2013.1
Haghighi M (2010) Laboratory methods of fish haematology. Aquaculture Science Publications, Tehran, Iran. pp. 29–71.
Haghighi M and Rohani MS (2013) The effect of powered ginger (Zingiber officinale) on the haematological and immunological parameters of rainbow trout Oncorrrhychus mykiss. Journal of Medicinal Plant and Herbal Therapy Research 1: 8–12.
Heidarieh M, Mirvaghefi AR, Sepahi A, Shiekhzadeh N and Shahbazfar AA (2013) Effects of dietary aloe vera on growth performance, skin and gastrointestine morphology in rainbow trout, Oncorhynchus mykiss. Turkish Journal of Fisheries and Aquatic Sciences 13: 361–373.
Immanuel G, Citarasu T, Sivaram V, Micheal Babu M and Palavesam A (2007) Delivery of HUFA, probionts and biomedicine through bioencapsulated Artemia as a means to enhance the growth and survival and reduce the pathogenesity in shrimp Penaeus monodon postlarvae. Aquaculture International 15(2): 137–152.
Ivanova NT (1983) Atlas of fish blood cells, LPP, Moscow, Russia. 80 pp.
Klontz GW and Smith LS (1986) Methods of using fish as biological research subjects. In methods of animal experimentation Vol. iii (Grey WI, ed.). Academic Press, New York. pp. 323–383.
Lee JK, Lee MK, Yun YP, Kim Y, Kim JS, Kim YS, Kim K, Han SS and Lee CK (2001) Acemannan purified from aloe vera induces phenotypic and functional maturation of immature dendritic cells. International Immunopharmacology 1(7): 1275–1284.
Mmereole EUC (2011) Evaluation of the dietary inclusion of aloe vera as an alternative to antibiotic growth promoter in broiler production. Pakistan Journal of Nutrition 10: 1–5.
Mohsin ABM, Reza MS and Galib SM (2012) Uses of chemicals in aquaculture ponds in Rajshahi district, Bangladesh. Bangladesh Journal of Progressive Science and Technology X(I): 61–64.
Muhammad-Jameel AK, Sohail, HK, Salma M, Syeda, SG, Jamila S, Farouq H, Mehdi H and Muhammad A (2014) Effect of Dietary supplementation of Aloe vera leaves on growth performance and immunity of Fayoumi Chicks. Pakistan Journal of Nutrition 13(4): 191–195.
Obasa SO and Faturoti EO (2001) Growth response and serum component and yield of the African bony tongue (Heterotis niloticus) fed varying dietary crude protein level. ASSET Series A 1(2): 97–104.
Odo BI, Ekenyem BU and Nwamo AC (2010) Effects of aloe vera as leaf protein concentrate on growth performance of cockerels. International Journal of Poultry Science 9: 426–428.
Oyawoye EO and Ogunkunle M (1998) Physiological and biochemical effects of raw Jack beans on broilers. Proceedings of Annual Conference, Nigeria Society of Animal Production 23: 141–142.
Pietse JJ, Smith GL, van Viiet KJ, Schoobe HJ and Hattingh J (1981) Some blood parameters of the Chinese grass carp, Ctenopharygodon idella (Valenciennes). South African Journal of Zoology 16(2): 124–126.
Plaskett LG (1988) The health and medical uses of aloe vera. Life Sciences Press.
Qiao J, Li HH, Zheng Ch J, Feng ZY and Wang W (2013) Dietary supplementation with aloe vera polysaccharide enhances the growth performance and immune function of weaned piglets. Journal of Animal Feed Science 22: 329–334.
Rainza-paiva MJT, Ishikawa CM, Das-Eiras, AA and Felizardo NN (2000) Haematological analysis of Chara Pseudoplatystoma fasciatum in captivity Aqua Responsible aquaculture in the new millennium. European Society Special Publication, Nice, France. 28: 590
Rao YV, Das J, Pradhana BK and Chakrabarthi R (2006) Effect of Achyranthes aspera on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Shell Fish Immunology 20: 263–273.
Rapatsa MM and Moyo NAG (2014) Effect of Moringer oleifera on the histology, haematology and growth of Oreochromis mossambicus in aquadams fertilized with chicken manure in South Africa. African Journal of Aquatic Science 39(3): 295–303.
Samuelson A (2007) Textbook of veterinary histology. Elsevier Health Sciences Publishing Company, London, United Kingdom. 560 pp.
Schaperclaus W (1991) Fish diseases. Oxonian Press Pvt. Ltd., New Delhi. 1398 pp.
Sotolu AO and Faturoti EO (2009) Growth performance and haematology of Clarias gariepinus fed varying inclusions of Leucaena laucocephala leaf meal. Revista UDO Agricola 9(4): 979–985.
Szende B and Suba Z (1999) Introduction to histopathology. Medicinal Publishing, Budapest. 292 pp.
Vogler BK and Ernst E (1999) Aloe vera: a systematic review of its clinical effectiveness. British Journal of General Practice 48: 832–828.
Windisch W, Schedle K, Plitzner C and Kroismayr A (2008) Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science 86: 140–148.
Zomrawi WB, Abdel Atti KHA and Mahala AG (2011) Effect of ginger root powder supplementation on broiler chick performance, blood and serum constituents, Online Journal of Animal and Feed Research 1(6): 457–460.
