Inclusion of cricket (Gryllus bimaculatus) meal in African catfish (Clarias gariepinus) feed influences disease resistance
In our present study, we observed the effect of cricket meal (CM) on disease resistance of African catfish. Fish were fed diets containing 350 g kg–1 and 400 g kg–1 of CM and 350 g kg–1 of fishmeal as control. The fish were divided into triplicates treatments of ten fish per replicate, weighed 22.5±0.6 g and fed with experimental diets for 40 days before being challenged against Aeromonas hydrophila. Relative percentage of survival (RPS) was recorded over 12 days post-challenge. White blood cell count, total protein, globulin and lysozyme showed significantly increasing levels in those fed with cricket meal diet compared to the control group. Mortalities at 12-day post-challenge significantly decreased to 30% (RPS: 66.7%) and 27% (RPS: 70%) for 35% and 40% CM respectively compared to 90% mortality in control group. Intestines and liver tissues of infected fish were dissected for pathogenic confirmation. The intestines of control diet showed the highest bacterial load (58.2×107 cfu g–1) compared to CM diets. The current study indicates that dietary CM could enhance the innate immune system and disease resistance of African catfish.
Ahamad B, Punniamurthy D, Kumar N, Malmarugan V, Suresh R and Ranganathan V (2013) Outbreak of bacterial haemorrhagic septicaemia in freshwater carps in Thanjavur region of Tamil Nadu. Proceedings of the national seminar on current perspectives in Biological Sciences (NSOCPIBS–2012).
Alderman D and Hastings T (1998) Antibiotic use in aquaculture: development of antibiotic resistance–potential for consumer health risks. International Journal of Food Science & Technology 33(2): 139–155.
Amend DF and McDowell T (1983) Current problems in the control of channel catfish virus. Journal of World Mariculture Society 14(1‐4): 261–267.
Anyanwu MU, Chah KF and Shoyinka VS (2015) Evaluation of pathogenicity of motile Aeromonas species in African catfish. International Journal of Fisheries and Aquatic Studies 2(3): 93–98.
AOAC (2003) Official methods of analysis, 17th edition, 2nd revision. Association of Official Analytical Chemists., Virginia, USA.
APHA (1992) Standard Methods for the Examination of Water and Wastewater, 18th edition. American Public Health Association, Washington DC, USA.
Barman D, Nen P, Mandal SC and Kumar V (2013) Immunostimulants for aquaculture health management. Journal of Marine Science: Research Development 3(3): 134.
Barroso FG, de Haro C, Sánchez-Muros M-J, Venegas E, Martínez-Sánchez A and Pérez-Bañón C (2014) The potential of various insect species for use as food for fish. Aquaculture 422: 193–201.
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry 72(1): 248–254.
Choudhury D, Pal A, Sahu N, Kumar S, Das S and Mukherjee S (2005) Dietary yeast RNA supplementation reduces mortality by Aeromonas hydrophila in rohu (Labeo rohita L.) juveniles. Fish and Shellfish Immunology 19(3): 281–291.
Dautremepuits C, Paris-Palacios S, Betoulle S and Vernet G (2004) Modulation in hepatic and head kidney parameters of carp (Cyprinus carpio L.) induced by copper and chitosan. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology 137(4): 325–333.
Depaola A, Peeler JT and Rodrick GE (1995) Effect of oxytetracycline-medicated feed on antibiotic resistance of gram-negative bacteria in catfish ponds. Applied and Environmental Microbiology 61(6): 2335–2340.
DOF (2014) Fisheries statistics of 2014. Department of Fisheries Malaysia. Available at www.dof.gov.my/fishery-statistics
Doumas BT, Watson WA and Biggs HG (1971) Albumin standards and the measurement of serum albumin with bromcresol green. Clinica Chimica Acta 31(1): 87–96.
Ellis A (1999) Immunity to bacteria in fish. Fish and Shellfish Immunology 9(4): 291–308.
Engel P and Moran NA (2013) The gut microbiota of insects–diversity in structure and function. FEMS microbiology Reviews 37(5): 699–735.
Esteban MA, Cuesta A, Ortuno J and Meseguer J (2001) Immunomodulatory effects of dietary intake of chitin on gilthead seabream (Sparus aurata L.) innate immune system. Fish and Shellfish Immunology 11: 303–305.
Galib SM, Mohsin ABM, Chaki N, Fahad MFH, Haque SMM (2013) An overview of the traditional rice-prawn-fish farming in Kalia of Narail district, Bangladesh. Journal of Fisheries 1(1): 1–6.
Gannam AL and Schrock RM (1999) Immunostimulants in fish diets. Journal of Applied Aquaculture 9(4): 53–89.
Gasco L, Henry M, Piccolo G, Marono S, Gai F, Renna M, Lussiana C, Antonopoulou E, Mola P and Chatzifotis S (2016) Tenebrio molitor meal in diets for European sea bass (Dicentrarchus labrax L.) juveniles: growth performance, whole body composition and in vivo apparent digestibility. Animal Feed Science and Technology 220: 34–45.
Gopalakannan A and Venkatesan A (2006) Immunomodulatory effect of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaculture 255: 179–187.
Henry M, Gasco L, Piccolo G and Fountoulaki E (2015) Review on the use of insects in the diet of farmed fish: past and future. Animal Feed Science and Technology 203: 1–22.
Hossain MF, Rahman MM and Sayed M (2013) Experimental infection of indigenous climbing perch Anabas testudineus with Aeromonas hydrophila bacteria. Progressive Agriculture 22(1–2): 105–114.
Ido A, Iwai T, Ito K, Ohta T, Mizushige T, Kishida T, Miura C and Miura T (2015) Dietary effects of housefly (Musca domestica) (Diptera: Muscidae) pupae on the growth performance and the resistance against bacterial pathogen in red sea bream (Pagrus major) (Perciformes: Sparidae). Applied Entomology and Zoology 50(2): 213–221.
Jabir MDAR, Razak SA and Sabaratnam V (2012) Nutritive potential and utilization of super worm (Zophobas morio) meal in the diet of Nile tilapia (Oreochromis niloticus) juvenile. African Journal of Biotechnology 11(24): 6592–6598.
Jadhav VS, Khan SI, Girkar MM and Gitte MJ (2006) The role of immunostimulants in fish and shrimp aquaculture. Aquaculture Asia 11(3): 24.
Janda JM and Abbott SL (2010) The genus Aeromonas: taxonomy, pathogenicity, and infection. Clinical Microbiology Reviews 23(1): 35–73.
Ji H, Zhang JL, Huang JQ, Cheng XF and Liu C (2015) Effect of replacement of dietary fish meal with silkworm pupae meal on growth performance, body composition, intestinal protease activity and health status in juvenile Jian carp (Cyprinus carpio var. Jian). Aquaculture Research 46(5): 1209–1221.
Kawakami H, Shinohara N and Sakai M (1998) The non-specific immunostimulation and adjuvant effects of Vibrio anguillarum bacterin, M-glucan, chitin and freund's complete adjuvant against Pasteurella piscicida infection in yellowtail. Fish Pathology 33(4): 287–292.
Kumar BN, Murthy HS, Patil P, Doddamani PL and Patil R (2015) Enhanced immune response and resistance to white tail disease in chitin-diet fed freshwater prawn, Macrobrachium rosenbergii. Aquaculture Reports 2: 34–38.
Kusdarwati R, Kurniawan H and Prayogi YT (2017) Isolation and identification of Aeromonas hydrophila and Saprolegnia sp. on catfish (Clarias gariepinus) in floating cages in Bozem Moro Krembangan Surabaya. IOP Conference Series: Earth and Environmental Science, IOP Publishing.
Laith AR and Najiah M (2013) Aeromonas hydrophila: antimicrobial susceptibility and histopathology of isolates from diseased catfish, Clarias gariepinus (Burchell). Journal of Aquaculture Research & Development 5: 215.
Law M (2001) Differential diagnosis of ulcerative lesions in fish. Environmental Health Perspectives 109(Suppl 5): 681–686.
Mari LSS, Jagruthi C, Anbazahan SM, Yogeshwari G, Thirumurugan R, Arockiaraj J, Mariappan P, Balasundaram C and Harikrishnan R (2014) Protective effect of chitin and chitosan enriched diets on immunity and disease resistance in Cirrhina mrigala against Aphanomyces invadans. Fish and Shellfish Immunology 39(2): 378–385.
Marono S, Piccolo G, Loponte R, Di Meo C, Attia YA, Nizza A and Bovera F (2015) In vitro crude protein digestibility of Tenebrio molitor and Hermetia illucens insect meals and its correlation with chemical composition traits. Italian Journal of Animal Science 14: 338–343.
McPhearson RM, DePaola A, Zywno SR, Motes ML and Guarino AM (1991) Antibiotic resistance in gram-negative bacteria from cultured catfish and aquaculture ponds. Aquaculture 99(3): 203–211.
Misra SK, Bhadra RK, Pal S and Nair GB (1989) Growth of Aeromonas spp. on Butzler Campylobacter selective agar and evaluation of the agar for the primary isolation of Aeromonas spp. from clinical specimens. Journal of Clinical Microbiology 27(2): 346–347.
Mohan M, Bhanja S and Basade Y (2009) Performance of chitin incorporated diet on the indigenous Kumaon Himalayan fishes: snow trout, Schizothorax richardsonii (Gray) and golden mahseer, Tor putitora (Hamilton). Indian Journal of Fisheries 56(2): 135–137.
Mohsin ABM, Islam MN, Hossain MA and Galib SM (2012a) Cost-benefit analyses of carp polyculture in ponds: a survey study in Rajshahi and Natore districts of Bangladesh. Bangladesh Journal of Environmental Science 23: 103–107.
Mohsin ABM, Islam MN, Hossain MA and Galib SM (2012b) Constraints and prospects of carp production in Rajshahi and Natore districts, Bangladesh. University Journal of Zoology, Rajshahi University 31: 69–72.
Mostafa K, Islam M and Rashid MM (2008) Experimental pathogenesis of Aeromonas hydrophila bacteria in stinging catfish Heteropneustes fossilis. Bangladesh Journal of Fisheries Research 12(1): 27–33.
Ohta T, Ido A, Kusano K, Miura C and Miura T (2014) A novel polysaccharide in insects activates the innate immune system in mouse macrophage RAW264 cells. Plos One 9(12): e114823.
Oliveira SRD, Souza RTYBD, Brasil EM, Andrade JIAD, Nunes ÉDSS, Ono EA and Affonso EG (2011) LD50 of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus. Acta Amazonica 41(2): 321–326.
Palti Y, Tinman S, Cnaani A, Avidar Y, Ron M and Hulata G (1999) Comparative study of biochemical and non-specific immunological parameters in two tilapia species (Oreochromis aureus and O. mossambicus). Israeli Journal of Aquaculture Bamidgeh 51: 148–156.
Rahman M and Chowdhury M (1999) Incidence of ulcer disease in African catfish (Clarias gariepinus Burchell) and trial for its chemotherapy. Bangladesh Journal of Fisheries Research 3(2): 193–200.
Rashid MM, Honda K, Nakai T and Muroga K (1994) An ecological study on Edwardsiella tarda in flounder farms. Fish Pathology 29(4): 221–227.
Sakai M, Kamiya H, Ishii S, Atsuta S and Kobayashi M (1992) The immunostimulating effects of chitin in rainbow trout, Oncorhynchus mykiss. Diseases in Asian Aquaculture 1: 413–417.
Sarkar M and Rashid MM (2012) Pathogenicity of the bacterial isolate Aeromonas hydrophila to catfishes, carps and perch. Journal of Bangladesh Agriculture University 10(1): 157–161.
Schmidt AS, Bruun MS, Dalsgaard I, Pedersen K and Larsen JL (2000) Occurrence of antimicrobial resistance in fish-pathogenic and environmental bacteria associated with four Danish rainbow trout farms. Applied and Environmental Microbiology 66(11): 4908–4915.
Schulz C, Knaus U, Wirth M and Rennert B (2005) Effects of varying dietary fatty acid profile on growth performance, fatty acid, body and tissue composition of juvenile pike perch (Sander lucioperca). Aquaculture Nutrition 11(6): 403–413.
Shugar D (1952) The measurement of lysozyme activity and the ultra-violet inactivation of lysozyme. Biochimica et Biophysica Acta 8: 302–309.
Siwicki AK (1990) Immunostimulating influence of levamisole on nonspecific immunity in carp (Cyprinus carpio). Developmental and Comparative Immunology 13(1): 87–91.
Siwicki AK, Anderson DP and Rumsey GL (1994) Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Veterinary Immunology and Immunopathology 41(1): 125–139.
Skelton PH (2001) A complete guide to the freshwater fishes of southern Africa. Struik Publisher. 395 pp.
Taufek NM, Aspani F, Muin H, Raji AA, Razak SA and Alias Z (2016b) The effect of dietary cricket meal (Gryllus bimaculatus) on growth performance, antioxidant enzyme activities, and haematological response of African catfish (Clarias gariepinus). Fish Physiology and Biochemistry 42(4): 1143–1155.
Taufek NM, Muin H, Raji AA, Razak SA, Yusof HM and Alias Z (2016a). Apparent digestibility coefficients and amino acid availability of cricket meal, Gryllus bimaculatus, and fishmeal in African catfish, Clarias gariepinus diet. Journal of the World Aquaculture Society 47(6): 798–805.
Thanikachalam K, Kasi M and Rathinam X (2010) Effect of garlic peel on growth, hematological parameters and disease resistance against Aeromonas hydrophila in African catfish Clarias gariepinus (Bloch) fingerlings. Asian Pacific Journal of Tropical Medicine 3(8): 614–618.
Thomas J, Jerobin J, Seelan TSJ, Thanigaivel S, Vijayakumar S, Mukherjee A and Chandrasekaran N (2013) Studies on pathogenecity of Aeromonas salmonicida in catfish Clarias batrachus and control measures by neem nanoemulsion. Aquaculture 396: 71–75.
Van Huis A, Van Itterbeeck J, Klunder H, Mertens E, Halloran A, Muir G and Vantomme P (2013) Edible insects—future prospects for food and feed security. FAO, Rome.
Wang D, Zhai SW, Zhang CX, Bai YY, An SH and Xu YN (2005) Evaluation on nutritional value of field crickets as a poultry feedstuff. Asian Australian Journal of Animal Science 18: 667–670.
Wang S-H and Chen J-C (2005) The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp Litopenaeus vannamei. Fish and Shellfish Immunology 19(3): 191–204.
Wilson RP and Moreau Y (1996) Nutrient requirements of catfishes (Siluroidei). Aquatic Living Resources 9: 103–111.
Yixiang M, Jianhua Y, Jinyun Z, Xianping Y, Xia W, Chenglong S and Pei L (2013) The Influence of maggot meal and l-carnitine on growth, immunity, antioxidant indices and disease resistance of black carp (Mylopharyngodon piceus). Journal of the Chinese Cereals and Oils Association 2: 1–9.