Genetic variation among three wild populations of stinging catfish (Heteropneustes fossilis) by allozyme electrophoresis
Stinging catfish (Heteropneustes fossilis) is one of the most popular indigenous catfish having considerable potential for aquaculture and commercial importance in Bangladesh. With a view to assessing the genetic status of H. fossilis, three samples Chalan Beel (Pabna), Burungi Beel (Jamalpur) and Bagapura Beel (Mymensingh) were analyzed. For genetic variation study, five enzymes (LDH, EST, MDH, PGM and GPI) were used encoded by eight loci of which three were polymorphic (Mdh-1*, Est-1* and Gpi-1*). The highest mean proportion of polymorphic loci, mean number of allele and the mean proportion of heterozygous loci per individual of the Chalan Beel population were observed (25.00%, 1.250 and 6.250%, respectively). The highest gene flow (33.5) and lowest population differentiation (0.0074) found in Burungi Beel-Bagapura Beel indicated the close relationship among them. In the Nei’s UPGMA dendrogram, the Chalan Beel population formed one cluster by the genetic distance of 0.0371 and the other cluster was formed by Burungi Beel and Bagapura Beel populations (D=0.003). The results suggested that a considerable genetic variation is maintained among the natural H. fossilis populations.
Akand MA, Hasan MR and Habib MAB (1991) Utilization of carbohydrate and lipid as dietary energy sources by stringing catfish Heteropneustes fossilis (Bloch). In: Fish nutrition research in Asia. Proceedings of the Fourth Asian Fish Nutrition Workshop. ed. De Silva S.S. Asian Fisheries Society, Manila, Philippines. pp. 93-100.
Alam MA, Akanda MSH, Khan MMR and Alam MS (2002) Comparison of genetic variability between a hatchery and a river population of rohu (Labeo rohita) by allozyme electrophoresis. Pakistan Journal of Biological Science 5(9): 959-961.
Allendorf FW and Ryman N (1986) Genetic management of hatchery stocks. In: N. Ryman and F. Utter (eds.), Population Genetics and Fishery Management. Washington Sea Grant Program, University of Washington Press, Seattle. pp. 141-158.
Ayala FJ (1975) Genetic differentiation during the speciation process. Evolution Biology 8: 1-78.
Barua S, Alam MS, Khan MMR and Simonsen V (2004) Genetic Variation in Four Hatchery Populations of Thai Pangas, Pangasius hypophthalmus of Mymensingh Region in Bangladesh Using Allozyme Marker. Pakistan Journal of Biological Science 7(2): 144-149.
Eunus ATM (2004) Investigation of allozymeic variation in Thai pangas (Pangasius hypophthalmus) sampled from four hatcheries of Bogra. M.S. Thesis. Department of Fisheries Biology and Genetics, Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh. 38 pp.
Grobler JP, Hoffman LC and Prinsloo JF (1997) A comparison of allozyme heterozygosity and life history variables in four strains of African catfish (Clarias gariepinus). South African Journal of Aquaculture Science 23(1): 31-41.
Hanzawa H, Taniguchi N and Numachi K (1988) Geographical differentiation in populations of Japanese dace Triborodon hakonenesis deduced from allozyme variation. Zoological Science 5: 449-461.
Hardjamulia A, Sukadi F, Subagyo and Gustiano R (2001) Aquaculture genetics research in Indonesia. In: Fish genetics research in member countries and institutions of the International Network on Genetics in Aquaculture. eds. Gupta, M.V. and Acosta, B.O. ICLARM Conf. Proc. pp. 51- 59.
Khan MMR (1999) Electrophoretic study of allozyme gene markers for Japanese loach Misgurnusanguillicaudatus (Cobitidae). Bangladesh Journal of Fisheries 22(1): 87-97.
Khan MR and Arai K (2000) Allozyme variation and genetic differentiation in the loach Misgurnus anguillicaudatus. Fisheries Science 66(2): 211-222. DOI: 10.1046/j.1444-2906.2000.00037.x
Kirpichnikov VS (1992) Adaptive nature of intrapopulational biochemical polymorphism in fish. Journal of Fish Biology 40(1): 1-16. DOI: 10.1111/j.1095-8649.1992.tb02548.x
Lal KK, Singh RK, Mohindra V, Singh B. and Ponniah AG (2003) Genetic make up of exotic catfish Clarias gariepinus in India. Asian Fisheries Science 16:229-234.
Leary RF and Brooke HE (1990) Starch gel electrophoresis and species distinctions. In: Methods for Fish Biology. Eds. Schreck, C.B. and Moyle, P.B. American Fisheries Society, Bathesda, Maryland, USA. 141-170.
Leesa-Nga SN, Siraj SS, Daud SK, Sodsuk PK, Tan SG, Soduk S (2000) Biochemical polymorphism in yellow catfish, Mystus nemurus (C&V), from Thailand. Biochemical Genetics 38(3-4): 77-85.
Lewontin RC (1974) The Genetic basis of evolutionary change. Columbia University Press, New York.
Lewontin RC and Hubby JL (1966) A molecular approach to the study of genic heterozygosity in natural populations. II amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics 54: 595-609.
Muneer PM, Gopalakrishnan A, Lal KK and Mohindra V (2007) Population genetic structure of endemic and endangered yellow catfish, Horabagrus brachysoma, using allozyme markers. Biochemical Genetics 45(9-10): 637-645.
Na-Nakorn U, Hara M, Taniguchi N. and Seki S (1998) Isozymes variation of Clarias macrocephalus from four locations in Thailand. Fisheries Science 64(4): 526-530.
Nasren S (2007) Study of population genetics structure of the stinging catfish (Heteropneustes fossilis Bloch) by microsatellite DNA marker. MS thesis. Department of Fisheries Biology and Genetics. Faculty of Fisheries, Bangladesh Agricultural University, Mymensingh, Bangladesh. 46 pp.
Nei M (1972) Genetic distance between populations. American Naturalist 106: 283-292.
Nei M (1975) Molecular Population Genetics and Evolution. North-Holland, Amsterdam. 288 pp.
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
Nevo E (1978) Genetic variation in natural populations: patterns and theory. Theoretical Population Biology 13: 121-171.
Nevo E, Beiles A and Ben-Shlomo R (1984) The evolutionary significance of genetic diversity, ecological, demographic and life history correlates. Evolution 53: 13-213.
Pouyaud L, Gustiano R and Legendre M (1998) Phylogenetic relationships among pangasiid catfish species (Siluriformes, Pangasiidae) and new insights on their zoogeography. Proc. workshop Catfish Asia project, Can Tho University, Vietnam. pp. 49-56.
Reina J, Martinez G, Amores A and Alvarez MC (1994). Interspecific genetic differentiation in Western Mediterranean sparid fish. Aquaculture 125(1-2): 47-57.
Senanan W, Kapuscinski AR, Na-Nakorn U and Miller LM (2004) Genetic impacts of hybrid catfish farming (Clarias macrocephalus, C. gariepinus) on native catfish populations in central Thailand. Aquaculture 235(1-4): 167-184. DOI: 10.1016/j.aquaculture.2003.08.020
Shahid M (2004) Investigation of morphological and allozyme variation in Thai pangas (Pangasius hypophthalmus) sampled from four hatcheries of Mymensingh. MS thesis. Department of Fisheries Biology and Genetics. Faculty of Fisheries. Bangladesh Agricultural University, Mymensingh. 56 pp.
Shimizu T, Taniguchi N and Mizuno N (1993) An electrophoretic study of genetic differentiation of a Japanese freshwater goby, Rhinigobius flumineus. Japanese Journal of Ichthyology 39: 329-343.
Sumantadinata K and Taniguchi N (1990) Comparison of electrophoretic allele frequencies and genetic variability of common carp stocks from Indonesia and Japan. Aquaculture 88: 263-271.
Triantafyllidis A, Ozouf-Costaz C, Rab P, Suciu R and Karakousis Y (1999) Allozyme variation in European silurid catfishes, Silurus glanis and Silurus aristotelis. Biochemical Systematics Ecology 27(5): 487-498. DOI: 10.1016/S0305-1978(98)00114-8
Utter FM (1991) Biochemical genetics and fishery management: an historical perspective. Journal of Fisheries Biology 39: 1-20.
Wright S (1978) Evolution and the Genetics of Populations, Vol. 4. Variability within and among natural populations. University of Chicago Press, Chicago.
Yeh FC, Yang RC and Boyle T (1999) POPGENE VERSION 1.31: Microsoft Windows-based Freeware for Population Genetic Analysis. ftp://ftp.microsoft.com/Softlib/HPGL.EXE.
Zawadzki C H, Reis RE and Renesto E (2000) Allozyme discrimination of three species of Loricariichthys (Siluriformes: Loricariidae) from Southern Brazil. Revue suisse de Zoologie (abstract).