Oxytetracycline resistant bacteria in Clarias gariepinus and Clarias batrachus larvae and the environment

Goondi Bharathkumar, Thangapalam Jawahar Abraham

Abstract


Antibiotics and several other chemicals are used in catfish aquaculture as a remedy for various diseases. Reports on the distribution of different groups of bacteria especially the antibiotic resistant bacteria in catfish nursery system are scanty. The present study quantified motile aeromonads, pseudomonads and antibiotic (oxytetracycline) resistant bacteria in Clarias gariepinus and C. batrachus larvae and the rearing water from five catfish nurseries of West Bengal, India between March 2007 and July 2007. The C. gariepinus and C. batrachus larvae recorded mean counts of log 7.26±0.18/g and log 6.24±0.64/g, log 6.37±0.41/g and log 4.93±0.51/g, log 5.46±0.22/g and log 4.38±0.53/g and, log 6.27±0.64/g and log 5.57±0.12/g, respectively for total viable bacteria (TVCs), motile aeromonads (MACs), presumptive pseudomonads (PPCs) and oxytetracycline resistant bacteria (ARBCs). The levels of TVCs, MACs, PPCs and ARBCs were significantly high in C. gariepinus than in C. batrachus larvae. The rearing water recorded comparatively low bacterial counts than in larvae. The difference between the TVCs of larvae and larval rearing water of C. gariepinus was statistically significant, where as it was insignificant for C. batrachus. The mean proportion of motile aeromonads of C. gariepinus larvae and rearing water was about 4-5 fold higher than in C. batrachus. The catfish nurseries recorded high prevalence of oxytetracycline resistant bacteria ranging from 3.58-35.71% in larval rearing water and 2.05-37.89% in larvae.

Keywords


Catfish larvae, Clarias gariepinus, Clarias batrachus, Motile aeromonads, Pseudomonads, Oxytetracycline resistant bacteria

Full Text:

PDF (196 KB)

References


Abraham TJ and Bharathkumar G (2009) Distribution of motile aeromonads, pseudomonads and oxytetracycline resistant bacteria in freshwater catfish, Pangasius pangasius hatcheries of West Bengal, India. Journal of Bio-Science 17: 13-20

APHA (1992) Compendium of Methods for the Microbiological Examination of Foods. C Vanderzent and DF Splittstoesser, Eds. American Public Health Association, Washington, DC

Austin B and Austin DA (2007) Bacterial Fish Pathogens: Diseases of Farmed and Wild Fish, 3rd (revised) edition. Springer-Praxis, Godalming

Bharathkumar G and Abraham TJ (2013) Prevalence of transferable oxytetracycline resistance factors in Aeromonas hydrophila in fish hatcheries. Fishery Technology 50: 324-330

Blanch AR, Alsina M, Simon and Jofre J (1997) Determination of bacteria associated with reared turbot (Scophthalmus maximus) larvae. Journal of Applied Microbiology 82: 729-734

Collins CH, Lyne PM and Grange JM (1989) Microbiological Methods. 6th Edition, Butterworths, London, UK.

Dang LT, Tu DT, Kondo H, Hirono I and Aoki T (2007) First determination of transferable antimicrobial resistance plasmids (R plasmids) in Edwardsiella ictaluri isolated from diseased freshwater catfish, Pangasius hypophthalmus (sauvage), in Vietnam. 13th International Conference of Fish and Shellfish Diseases, 17-21 September 2007, The European Association of Fish Pathologists, Grado, Italy. Abstract O-038, p. 47.

FAO (2014) The State of World Fisheries and Aquaculture. Food and Agriculture Organization of the United Nations. Rome. p. 223.

Husevag B, Lunestad BT, Johanneessen PJ, Enger O and Samuelson OB (1991). Simultaneous occurrence of Vibrio salmonicida and antibiotic-resistant bacteria in sediments at abandoned aquaculture sites. Journal of Fish Diseases 14: 631-640.

Kha NHN, Sarter S, Legarve T and Montet D (2006) Characterization of bacteria isolated from farmed catfish coming from Vietnam and study of their antibiotic resistance. In: Proc. International Workshop on Biotechnology in Agriculture, October 20-21, 2006, Nong Lam University, Ho Chi Minh City, Vietnam, pp. 103-108.

Lechevallier M, Seidler RJ and Evans TM (1980) Enumeration and characterization of standard plate count bacteria in chlorinated and raw water supplies. Applied Environmental Microbiology 40(5): 922-930

Mohanty BR and Sahoo PK (2007) Edwardsiellosis in fish: a brief review. Journal of Biosciences 32(7): 1331-1344

Nahiduzzaman M, Ehsan MA and Chowdhury BR (2000) Investigation on the occurrence of bacterial flora in hybrid catfish (Clarias hybrid) in a commercial fish farm. Pakistan Journal of Biological Sciences 3(1): 38-42

Ogbondeminu FS (1994) Commensal bacterial microflora associated with incubating eggs of Clarias angullaris in a tropical hatchery. Journal of Aquaculture in the Tropics 9: 151-156

Olafsen JA (2001) Interactions between fish larvae and bacteria in marine aquaculture. Aquaculture 200: 223-247

Palumbo SA, Maxino F, Williams AC, Buchanan RL and Thayer DW (1985) Starch-ampicillin agar for the quantitative detection of Aeromonas hydrophila. Applied Environmental Microbiology 50: 1027-1030

Sahoo PK, Mukherjee SC and Sahoo SK (1998) Aeromonas hydrophila versus Edwarsiella tarda: A pathoanatomical study in Clarias batrachus. Journal of Aquaculture 6: 57-66

Sahoo SK, Giri SS, Chandra S and Sahu AK (2007) Spawning performance and egg quality of Asian catfish Clarias batrachus (Linn.) at various doses of human chorionic gonadotropin (HCG) injection and latency periods during spawning induction. Aquaculture 266(1-4): 289-292.

Sowunmi AA, Okunubi MA and Efuntoye MO (2008) Occurrence of bacteria in gills and buccal cavity of Clarias gariepinus (Burchell, 1822) and Tilapia zillii (Gervais) from Lekki lagoon, Southwest Nigeria. World Journal of Biological Resources 1(1): 14-17.

Subagja J, Slembrouck J, Hung LT and Legendre M (1999) Larval rearing of an Asian catfish Pangasius hypophthalmus (Siluroidei, Pangasiidae): Analysis of precocious mortality and proposition of appropriate treatments. Aquatic Living Resources 12: 37-44.




DOI: http://dx.doi.org/10.17017/jfish.v3i1.2015.48

Refbacks

  • There are currently no refbacks.





| Intellectual property: The Author(s) | Publication property: BdFISH | All contents of this site are protected under CC BY-NC-SA license. |
| The opinions expressed herein are those of the author(s) and do not necessarily reflect the views of BdFISH. |

| Editor-in-Chief: Professor MN Islam | Editorial Team | Principal Contact |

| Note: If you unable to register as a new author or submit a manuscript through user panel, you may submit your manuscript through our editorial email (editor.journal@bdfish.org). |

| BdFISH Journal: The Scientific Publication of BdFISH (Bangladesh Fisheries Information Share Home) |