Effects of different background colors on adult spawning, egg hatchability, and larval survival of zebrafish (Danio rerio)

  • Marwin B. Dela Cruz Fisheries and Marine Sciences Department, Cavite State University – Naic Campus, Naic, Cavite 4110, Philippines
Keywords: Breeding, environment color, hatching rate, ornamental fish, spawn


Background color has significant effects on the reproductive and survival performance of fish species. While zebrafish (Danio rerio) is a popular species in laboratory and recreational aquaculture, very little has been known about its background color preference. Thus, this study evaluated the effects of different colors of aquaria on the spawning, hatching, and survival of this species. The study found that zebrafish do not have color preference for spawning. Though red and black backgrounds are statistically comparable, these colors showed significantly higher (P < 0.05) hatching and survival rates than other colors used in this study. Furthermore, performance of red was higher than black but it was not statistically significant. This study reveals that, among all colors evaluated, red is the most suitable for hatching of zebrafish eggs and survival of its larvae whereas, yellow and transparent should be avoided.


Amsterdam A, Hopkins N (2006) Mutagenesis strategies in zebra fish for identifying genes involved in development and disease. Trends in Genetics 22: 473–478.

Banan A, Kalbassi MR, Bahmani M, Sadati MAY (2011) Effects of colored light and tank color on growth indices and some physiological parameters of juvenile beluga (Huso huso). Journal of Applied Ichthyology 27: 565–570.

Barcellos LJG, Kreutz FRLC, Quevedo RM, Santos da Rosa JG, Koakoski G, Centenaro L, Pottker E (2009) Influence of color background and shelter availability on jundiá (Rhamdia quelen) stress response. Aquaculture 288: 51–56.

Brian O (2015) Effect of tank background colour on the hatchability of O. niloticus eggs and survival of fry. International Journal of Fisheries and Aquatic Studies 2(6): 81–86.

Elnwishy N, Sabri D, Nwonwu F (2012) The effect of difference in environmental colors on Nile tilapia (Oreochromis niloticus) production efficiency. International Journal of Agriculture and Biology 14(4): 516–520.

Galib SM, Mohsin ABM (2011) Exotic ornamental fishes of Bangladesh: past and present. LAP Lambert Academic Publishing, Germany.

Imanpoor MR, Abdollah M (2011) Effects of tank color on growth, stress response and skin color of juvenile Caspian kutum, Rtilus frisii Kutum. Global Veterinaria 6(2): 118–125.

Kesbic OS, Yigit M, Acar U (2017) Effects of tank color on growth performance and nitrogen excretion of European seabass (Dicentrarchus labrax) juveniles. Proceedings of the National Academy of Science India, Section B Biological Science 86(1): 205–210.

Kucharczyk D, Stępień P, Nowosad J, Kupren K, Targońska K, Kujawa R (2018) The optimization of wide-type zebrafish, Danio rerio (Hamilton, 1822) reproduction in low temperatures under controlled conditions. Turkish Journal of Fisheries and Aquatic Sciences 18: 49–55.

Lawrence C (2007) The husbandry of zebrafish (Danio rerio): a review. Aquaculture 269: 1–20.

Liew WC, Bartfai R, Lim Z, Sreenivasan R, Siegfried KR, Orban L (2012) Polygenic sex determination system in zebra fish. PLoS ONE 7(4): 1–12.

Manliclic ADC, Corpuz MNC, Vera Cruz EM (2018) Optimum conditioning period before packing, salt-treated water, and blue background color improved the survival of Nile tilapia (Oreochromis niloticus L.) fingerlings during transport. The Philippine Agricultural Scientist 101(1): 76–83.

McLean E, Cotter P, Thain C, King N (2008) Tank color impacts performance of cultured fish. Ribarstvo 66(2): 43–54.

Pedreira MM, Sipaúba-Tavares LH (2001) Effect of light green and dark brown colored tanks on survival rates and development of tambaqui larvae, Colossoma macropomum (Osteichthyes, Serrasalmidae). Acta Scientiarum Biological Sciences 23(2): 521–525.

Sabri DM, Elnwishy N, Nwonwu F (2012) Effect of environmental color on the behavioral and physiological response of Nile tilapia, Oreochromis niloticus. Global Journal of Science Frontier Research Biological Sciences 12(4): 1–12

Strand A, Alanara A, Staffan F, Magnhagen C (2007) Effects of tank colour and light intensity on feed intake, growth rate and energy expenditure of juvenile Eurasian perch, Perca fluviatilis L. Aquaculture 272 (1–4): 312–318.

Takeshita A, Soyano K (2008) Effects of light intensity and color of rearing tank on cannibalism in the juvenile orange-spotted grouper (Epinephelus coioides). Aquaculture Science 56(2): 175–180.

Tamazouzt L, Chatain B, Fontaine P (2000) Tank wall color and light level affect growth and survival of Eurasian perch larvae (Perca fluviatilis L.). Aquaculture 182 (1–2): 85–90.

Tavares B, Lopes SS (2013) The Importance of zebra fish in biomedical research. Acta Medica Portuguesa 26(5): 583–592.

Volpato GL, Barreto RE (2001) Environmental blue light prevents stress in the fish Nile tilapia. Brazilian Journal of Medical and Biological Research 34: 1041–1045.

Volpato GL, Duarte CRA, Luchiari AC (2004) Environmental color affects Nile tilapia reproduction. Brazilian Journal of Medical and Biological Research 37: 479–483.

Westerfield M (2000) The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th ed. University of Oregon Press, Eugene.

How to Cite
Dela Cruz, M. B. (2020). Effects of different background colors on adult spawning, egg hatchability, and larval survival of zebrafish (Danio rerio). Journal of Fisheries, 8(3), 891-894. Retrieved from http://journal.bdfish.org/index.php/fisheries/article/view/JFish20241