Formulation and characterization of Halymenia dilatata-based nori alternative through nutritional, sensory, structural, textural, and functional analyses
Abstract
The increasing demand for seaweed-based foods has created interest in developing sustainable alternatives to conventional Porphyra-based nori using tropical seaweed resources. This study developed a nori-like product from Halymenia dilatata and evaluated the effects of glycerin concentration and drying conditions on its nutritional, physicochemical, structural, and sensory properties. Four formulations were prepared using a constant seaweed-to-water ratio (1:10, w/v) with varying glycerin concentrations. The formulation containing 2.5% glycerin and dried at 55°C for 2 h produced sheets with the best pliability, uniformity, and handling characteristics. The optimized product contained low moisture (7.84%), moderate protein (11.62%), high dietary fibre (31.54%), and a mineral-rich ash content (14.87%), with appreciable levels of iron and zinc and a balanced sodium-to-potassium ratio. Sensory evaluation indicated good consumer acceptance for appearance, flavour, texture, and overall acceptability. Structural analyses confirmed the formation of a cohesive polysaccharide network, while biochemical and microbiological analyses demonstrated favourable quality, oxidative stability, and microbial safety. These findings demonstrated that H. dilatata could be successfully developed into a nutritious, safe, and acceptable nori-like product with physicochemical characteristics comparable to those of conventional nori, highlighting its potential as a sustainable tropical alternative for value-added seaweed-based food products.
References
AOAC (2016) Official methods of analysis of AOAC International, 20th edition. AOAC International. Gaithersburg, MD, USA.
Barth A (2007) Infrared spectroscopy of proteins. Biochim Biophys Acta Bioenerg 1767: 1073–1101.
Černá M (2011) Seaweed proteins and amino acids as nutraceuticals. Advances in Food and Nutrition Research 64: 297–312.
Cherry P, O’Hara C, Magee PJ, McSorley EM, Allsopp PJ (2019) Risks and benefits of consuming edible seaweeds. Nutrition Reviews 77(5): 307–329.
Cian RE, Drago SR, de Medina FS, Martínez-Augustin O (2014) Development of naturally activated edible films with antioxidant properties prepared from red seaweed Porphyra columbina biopolymers. Food Chemistry 146: 6–14.
Dawczynski C, Schubert R, Jahreis G (2007) Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chemistry 103(3): 891–899.
Erniati ZF, Prangdimurti ENDANG, Adawiyah DR, Priosoeryanto BP, Huda NURUL (2018) Chemical evaluation of a nori-like product (geluring) made from the mixture of Gelidium sp. and Ulva lactuca seaweeds. Current Research in Nutrition and Food Science 6(3): 664–671.
FAO (2013) Dietary protein quality evaluation in human nutrition (FAO Food and Nutrition Paper No. 92). Food and Agriculture Organization of the United Nations & World Health Organization, Rome.
Faris A, Liviawaty E, Andriani Y, Affrianto E (2019) Nori level of preference with mixed Sargassum sp. and Eucheuma spinosum seaweed as raw material. Asian Food Science Journal 11(2): 1–9.
Fenoradosoa TA, Delattre C, Laroche C, Wadouachi A, Dulong V, ... Michaud P (2009) Highly sulphated galactan from Halymenia durvillei (Halymeniales, Rhodophyta), a red seaweed of Madagascar marine coasts. International Journal of Biological Macromolecules 45(2): 140–145.
Fleurence J, Morançais M, Dumay J, Decottignies P, Turpin V, ... Jaouen P (2012) What are the prospects for using seaweed in human nutrition and for marine animals raised through aquaculture? Trends in Food science & Technology 27(1): 57–61.
Harrysson H, Krook JL, Larsson K, Tullberg C, Oerbekke A, ... Undeland I (2021) Effect of storage conditions on lipid oxidation, nutrient loss and colour of dried seaweeds, Porphyra umbilicalis and Ulva fenestrata, subjected to different pretreatments. Algal Research 56: 102295.
He FJ, MacGregor GA (2008) Beneficial effects of potassium on human health. Physiologia Plantarum 133(4): 725–735.
Ishida Y, Fujita T, Asai K (1981) New detection and separation method for amino acids by high-performance liquid chromatography. Journal of Chromatography A 204: 143–148.
Jacobs MB (1958) The chemical analysis of foods and food products. D. Van Nostrand Company, Inc., New York, NY, USA.
Jung H, Yoon WB, Matsukawa S (2022) Effect of moisture uptake on the texture of dried laver Porphyra (nori) studied by mechanical characterization and NMR measurements. Food Hydrocolloids 124: 107223.
Kjeldahl J (1983) New method for determining nitrogen in organic substances. Fresenius, Zeitschrift f. Anal. Chemie (Analytical and Bioanalytical Chemistry) 22: 366–382.
Krimm S, Bandekar J (1986) Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins. Advances in Protein Chemistry 38: 181–364.
Kurakake M, Itakura K, Nakano M (2021) Functional properties of nori seaweed (Pyropia yezoensis) with different quality and Ulva sp. Journal of Aquatic Food Product Technology 30(2): 188–195.
Lee H-j, Choi J-i, Choi, S-J (2012) Physiological activities and amino acid compositions of Korean dried laver Porphyra products. Korean Journal of Fisheries and Aquatic Sciences 45(5): 409–413.
Limonu M, Bait Y, Engelen A, Muhsin N (2024) Seaweed nori (Kappapycus alvarezi) physicochemical and organoleptic characteristics with moringa leaf fortification (Moringa oleifera LAM). Agrointek: Jurnal Teknologi Industri Pertanian 18(2): 360–372.
Listyaningrum RS, Hidayatulloh IS (2024) Sensory and chemical characteristics of nori from Indonesian seaweed added with breadfruit leaf extract and grass jelly. Agrointek: Jurnal Teknologi Industri Pertanian, 18(4): 1012–1018.
Loupatty VD (2015) Nori nutrient analysis from seaweed of Porphyra marcossi in Maluku Ocean. EKSAKTA: Journal of Sciences and Data Analysis 14(2): 34–48.
Løvdal T, Lunestad BT, Myrmel M, Rosnes JT, Skipnes D (2021) Microbiological food safety of seaweeds. Foods 10(11): 2719.
Mariotti F, Tomé D, Mirand PP (2008) Converting nitrogen into protein—beyond 6.25 and Jones' factors. Critical Reviews in Food Science and Nutrition 48(2): 177–184.
Meilgaard MC, Civille GV, Carr BT (2016) Sensory evaluation techniques fifth edition. CRC Press, Boca Raton, FL, USA.
Müller CM, Yamashita F, Laurindo JB (2008) Evaluation of the effects of glycerol and sorbitol concentration and water activity on the water barrier properties of cassava starch films through a solubility approach. Carbohydrate Polymers 72(1): 82–87.
Noda H (1993) Health benefits and nutritional properties of nori. Journal of Applied Phycology 5(2): 255–258.
Olley J, Lovern JA (1960) Phospholipid hydrolysis in cod flesh stored at various temperatures. Journal of the Science of Food and Agriculture 11(11): 644–652.
Ortiz J, Romero N, Robert P, Araya J, Lopez-Hernández J, ... Rios A (2006) Dietary fiber, amino acid, fatty acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea antarctica. Food Chemistry 99(1): 98–104.
Panjaitan TFC, Sabar Tumohom Panjaitan P, Pramono Adi C, Soeprijadi L (2021) Study of the use of Gracilaria sp from the Karawang area and Ulva lactuca as raw material making of nori. IOP Conference Series: Earth and Environmental Science 860(1): 012068.
Pereira L, Amado AM, Critchley AT, van de Velde F, Ribeiro-Claro PJA (2009) Identification of selected seaweed polysaccharides (phycocolloids) by vibrational spectroscopy (FTIR-ATR and FT-Raman). Food Hydrocolloids 23(7): 1903–1909.
Rondevaldova J, Quiao MA, Drabek O, Dajcl J, Dela Pena-Galanida GD, ... Kokoska L (2023) Mineral composition of seaweeds and seagrasses of the Philippines. Phycologia 62(3): 217–224.
Rupérez P (2002) Mineral content of edible marine seaweeds. Food Chemistry 79(1): 23–26.
Secci G, Parisi G (2016) From farm to fork: lipid oxidation in fish products. a review. Italian Journal of Animal Science 15: 124–136.
Sinurat E, Fransiska D, Livia (2021) The effect of addition glycerol against nori characterization from Gracilaria sp and Ulva sp seaweeds. IOP Conference Series: Earth and Environmental Science 715(1): 012054.
Sinurat E, Fransiska D, Utomo BSB, Subaryono, Nurhayati, Sihono (2022) Characteristics of nori-like product prepared from seaweeds growing in Indonesia. Journal of Aquatic Food Product Technology 31(6): 525–535.
Sinurat E, Sihono S, Fateha F, Supriyanto A, Suryaningrum TD, ... Waryanto W (2024) Optimization and characterization of nori-like product made from Ulva lactuca and Gracilaria changii using mixture design. Journal of Applied Phycology 36(4): 2343–2357.
Sravani K, Ganesan P, Balasundari S, Muralidharan N, Chrisolite B, Dhanapal K (2023) Effect of different drying methods on the nutritional, functional, and bioactive properties of Gracilaria edulis and Ulva lactuca. Journal of Coastal Research 39(5): 940–948.
Taboada MC, Millán R, Miguez MI (2013) Nutritional value of the marine algae wakame (Undaria pinnatifida) and nori (Porphyra purpurea) as food supplements. Journal of Applied Phycology 25(5): 1271–1276.
Vanderzant C, Splittstoesser DF (1992) Compendium of methods for the microbiological examination of foods, 3rd edition, American Public Health Association, Washington DC.
Wong KH, Cheung PC (2000) Nutritional evaluation of some subtropical red and green seaweeds: Part I—proximate composition, amino acid profiles and some physico-chemical properties. Food Chemistry 71(4): 475–482.
Xavier J, Jose J (2020) Study of mineral and nutritional composition of some seaweeds found along the coast of Gulf of Mannar, India. Plant Science Today 7(4): 631–637.
Yogarajalakshmi P, Poonguzhali TV (2023) Biochemical composition of H. dilatata and L. ceranoides from Indian coast, Tamil Nadu. International Journal of Innovative Research in Technology 10(7): 89–94.
Zeb A, Ullah F (2016) A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. Journal of Analytical Methods in Chemistry 2016(1): 9412767.
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