Molecular characterisation and genetic analysis of aerolysin and haemolysin in Aeromonas hydrophila isolated from diseased Labeo rohita by polymerase chain reaction

Fatima Sughra; Muhammad Hafeez-ur-Rehman; Farzana Abbas; Imran Altaf; Zohal Hassan; Ayesha Bhatti; Kashif Ali

doi:10.17017/j.fish.466

Fatima Sughra Department of Zoology, Division of Science and Technology, University of Education, Lahore, Pakistan
Muhammad Hafeez-ur-Rehman Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan
Farzana Abbas Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan
Imran Altaf Quality Operations and Research Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan
Zohal Hassan Quality Operations and Research Laboratory, University of Veterinary and Animal Sciences, Lahore, Pakistan
Ayesha Bhatti Department of Fisheries and Aquaculture, University of Veterinary and Animal Sciences, Lahore, Pakistan
Kashif Ali Department of Wildlife & Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan

DOI: https://doi.org/10.17017/j.fish.466

Keywords: aerolysin, Aeromonas hydrophila, DNA, haemolysin, polymerase chain reaction, sequencing

Abstract

The present study investigates pathogenicity of local Aeromonas hydrophila strains by molecular characterisation of two virulence factor genes: aerolysin (aerA) and haemolysin (Ahh1) using polymerase chain reaction (PCR) technique. Phenotypically identified presumptive Aeromonas isolates recovered from diseased Labeo rohita were genetically analysed using type-specific primers by amplifying 309 bp and 130 bp conserved regions of aerolysin and haemolysin genes respectively. The partial nucleotide sequences of aerA and Ahh1 were determined from representative strains in which aerA was confirmed in 75% isolates, whereas Ahh1 was confirmed in 50% isolates. The nucleotide blast results of the representative strains revealed close homology of 95% (aerolysin) and 97% (haemolysin) with published sequences.

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