Evaluation of extracellular enzyme-producing autochthonous gut bacteria in walking catfish, Clarias batrachus (L.)

Atrayee Dey; Koushik  Ghosh; Niladri  Hazra

doi:10.17017/j.fish.112

Atrayee Dey Entomology Research Unit, Department of Zoology, The University of Burdwan, West Bengal 713104, India
Koushik Ghosh Aquaculture Laboratory, Department of Zoology, The University of Burdwan, West Bengal 713104, India
Niladri Hazra Entomology Research Unit, Department of Zoology, The University of Burdwan, West Bengal 713104, India

DOI: https://doi.org/10.17017/j.fish.112

Keywords: Autochthonous bacteria, Bacillus, Clarias batrachus, extracellular enzymes, gut, 16S rRNA

Abstract

The present study was carried out to screen autochthonous gut bacteria in freshwater air breathing walking catfish, Clarias batrachus Linnaeus. Altogether, 100 extracellular enzyme-producing bacteria were isolated from the foregut (FG) and hindgut (HG) regions. Data were presented as log viable counts g^-1 gut (LVC). The occurrence of heterotrophic bacterial population was higher in the FG region (LVC = 8.25) than the HG (LVC= 7.3). Similarly, proteolytic, amylolytic and lipolytic bacteria in FG outnumbered (LVC=7.25, 6.77 and 5.23 respectively) the HG (LVC=6.38, 5.58 and 4.04 respectively). However, occurrence of cellulolytic bacteria in both, FG and HG was less (LVC=2.1 and 1.34 respectively) in comparison to the other extracellular enzyme-producing bacteria. Out of the 100 bacterial isolates, 22 isolates were primarily selected through qualitative assay of extracellular enzyme activities. Among them, 3 promising isolates were chosen as potent extracellular enzyme producers on the basis of cumulative scores (≥11) of the qualitative assay and quantitative enzyme assay. Maximum protease activity was revealed by the strain FG10 (201±2.40U), while FG43 exhibited maximum amylase (208.3±10.8U) and lipase (4.73±0.05U) activities. Among the strains isolated from the HG, the highest protease (188.3±1.2U), amylase (97.6±0.46U) and lipase (3.7±0.11U) activities were recorded with the strain HG01.

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